TFEC Polyclonal antibody

TFEC Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 13547-1-AP

Synonyms

bHLHe34, hTFEC L, TCFEC, TFE C, TFEC, TFECL, transcription factor EC, Transcription factor EC like

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Validation Data Gallery

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  WB analysis of mouse testis using 13547-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13547-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13547-1-AP

  IP result of anti-TFEC (IP:13547-1-AP, 4ug; Detection:13547-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, COLO 320 cells
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13547-1-AP targets TFEC in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFEC fusion protein Ag4061
• Full Name: transcription factor EC
• Calculated Molecular Weight: 347 aa, 39 kDa
• Observed Molecular Weight: 35-40 kDa, 65 kDa
• GenBank Accession Number: BC029891
• Gene Symbol: TFEC
• Gene ID (NCBI): 22797
• RRID: AB_2199629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. The expression of TFEC is mainly in fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells. 50kDa TFEC isoforms are produced by alternative splicing (PMID: 29303964, PMID: 11467950).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Weihao (Verified Customer) (02-03-2022)

Works great for mouse BMDM and human MDM, strong signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500