Validation Data Gallery
|Positive WB detected in||K-562 cells, human brain tissue, mouse spleen tissue, rat brain tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14480-1-AP targets TFE3 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TFE3 fusion protein Ag5863|
|Full Name||transcription factor binding to IGHM enhancer 3|
|Calculated molecular weight||62 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC026027|
|Gene ID (NCBI)||7030|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.
PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.
Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
Bioorg Med Chem
Terpenoids isolated from Chinese liverworts Lepidozia reptans and their anti-inflammatory activity.
Biochim Biophys Acta Gen Subj
Anti-cancer effect of marchantin C via inducing lung cancer cellular senescence associated with less secretory phenotype.
Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis