Anticorps Polyclonal de lapin anti-TCTN2

• Phare
• Validé par KD/KO

TCTN2 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 17053-1-AP

Synonymes

C12orf38, TCTN2, TECT2, Tectonic 2, tectonic family member 2

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Galerie de données de validation

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  IHC staining of human colon cancer using 17053-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17053-1-AP (TCTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17053-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17053-1-AP (TCTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17053-1-AP (TCTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17053-1-AP (TCTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

17053-1-AP cible TCTN2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCTN2 Protéine recombinante Ag10725
• Nom complet: tectonic family member 2
• Masse moléculaire calculée: 697 aa, 77 kDa
• Numéro d’acquisition GenBank: BC009112
• Symbole du gène: TCTN2
• Identification du gène (NCBI): 79867
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.

Publications

Species	Application	Title
human	WB,IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	WB	Cell; Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.; Authors - Sang Liyun L
human	WB,IF	Nat Cell Biol; CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.; Authors - Wang Won-Jing WJ
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
mouse	IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering
human	IF	J Cell Biol; Phosphorylation of CEP83 by TTBK2 is necessary for cilia initiation.; Authors - Chien-Hui Lo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jay (Verified Customer) (12-02-2019)

Methanol Fixation.Immunostaining result shows that Tctn2 localizes to centriole in ciliated NIH3T3 cell (Tctn2;red, Arl13b;green for primary cilia, DAPI;blue for nucleus).The antibody seems to have noisy background in cytoplasm.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse NIH3T3

TCTN2 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 (Cat no:17053-1-AP)

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