ATXN10 Polyclonal antibody

ATXN10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15693-1-AP

Synonyms

SCA10, E46L, Ataxin-10, ataxin 10

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Validation Data Gallery

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  WB analysis of human brain using 15693-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15693-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15693-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 15693-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15693-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 15693-1-AP

  IP result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 15693-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 15693-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15693-1-AP

  Immunofluorescent analysis of Hela cells, using ATXN10 antibody 15693-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, human kidney tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15693-1-AP targets ATXN10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATXN10 fusion protein Ag8259
• Full Name: ataxin 10
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC007508
• Gene Symbol: ATXN10
• Gene ID (NCBI): 25814
• RRID: AB_2061167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

Publications

Species	Application	Title
mouse	WB	Cell; Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.; Authors - Sang Liyun L
human	WB	J Neurochem; Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.; Authors - Robert J Andrew