human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15693-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human kidney tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15693-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human cerebellum using 15693-1-AP
human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15693-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 15693-1-AP
IP result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, human kidney tissue
Positive IP detected in
A431 cells
Positive IHC detected in
human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Hela cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.
human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15693-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15693-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human cerebellum using 15693-1-AP
human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15693-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary tumor using 15693-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary tumor using 15693-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of A431 using 15693-1-AP
IP result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.
IF/ICC Figures
IF Staining of HeLa using 15693-1-AP
Immunofluorescent analysis of Hela cells, using ATXN10 antibody 15693-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ATXN10 Polyclonal antibody
Catalog Number
15693-1-AP
Citations
3
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet