Anticorps Polyclonal de lapin anti-STK11/LKB1

• Phare
• Validé par KD/KO

STK11/LKB1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

45

Avis (1)

N° de cat : 10746-1-AP

Synonymes

LKB1, PJS, serine/threonine kinase 11, STK11, STK11/ LKB1

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Galerie de données de validation

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  WB analysis of HepG2 using 10746-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10746-1-AP (LKB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10746-1-AP

  IP result of anti-STK11/LKB1 (IP:10746-1-AP, 4ug; Detection:10746-1-AP 1:500) with HEK-293 cells lysate 2000ug.

• 

  IHC staining of human lung cancer using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10746-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10746-1-AP (STK11/LKB1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2,
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 39 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 6 publications below
• CoIP: See 4 publications below

Informations sur le produit

10746-1-AP cible STK11/LKB1 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STK11/LKB1 Protéine recombinante Ag1048
• Nom complet: serine/threonine kinase 11
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC007981
• Symbole du gène: LKB1
• Identification du gène (NCBI): 6794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.

Publications

Species	Application	Title
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
mouse	WB	EBioMedicine; Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure; Authors - Ke Qian
human	WB	EMBO J; CARS senses cysteine deprivation to activate AMPK for cell survival.; Authors - Mengqiu Yuan ; KD Validated
mouse	WB	Kidney Int; Rho-associated, coiled-coil-containing protein kinase 1 regulates development of diabetic kidney disease via modulation of fatty acid metabolism.; Authors - Yosuke Nagai
rat	CoIP	Aging Cell; Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway.; Authors - Juan Ji

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bogdan (Verified Customer) (11-29-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: nerve

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