CAB39L Polyclonal antibody

CAB39L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 16137-1-AP

Synonyms

Antigen MLAA 34, CAB39L, MO25 BETA, Mo25 like protein, MO2L, RP11 103J18.3

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Validation Data Gallery

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  WB analysis of K-562 using 16137-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16137-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 16137-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 16137-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16137-1-AP (CAB39L Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lymphoma using 16137-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16137-1-AP (CAB39L Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 16137-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAB39L antibody 16137-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, human spleen tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

16137-1-AP targets CAB39L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAB39L fusion protein Ag9181
• Full Name: calcium binding protein 39-like
• Calculated Molecular Weight: 337 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC010993
• Gene Symbol: CAB39L
• Gene ID (NCBI): 81617
• RRID: AB_2066394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Medicina (Kaunas); Bioinformatics Prediction and Experimental Verification Identify CAB39L as a Diagnostic and Prognostic Biomarker of Kidney Renal Clear Cell Carcinoma; Authors - Yunfei Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size. No additional background bands were observed around the size range. AWESOME!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, U2OS

CAB39L Antibody Western Blot, validation (1:1000 dilution) in 293T, U2OS (Cat no:16137-1-AP)