Tested Applications
| Positive WB detected in | K-562 cells, HEK-293 cells, human spleen tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16137-1-AP targets CAB39L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9181 Product name: Recombinant human CAB39L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-337 aa of BC010993 Sequence: MKKMPLFSKSHKNPAEIVKILKDNLAILEKQDKKTDKASEEVSKSLQAMKEILCGTNEKEPPTEAVAQLAQELYSSGLLVTLIADLQLIDFEEKKDVTQIFNNILRRQIGTRSPTVEYISAHPHILFMLLKGYEAPQIALRCGIMLRECIRHEPLAKIILFSNQFRDFFKYVELSTFDIASDAFATFKDLLTRHKVLVADFLEQNYDTIFEDYEKLLQSENYVTKRQSLKLLGELILDRHNFAIMTKYISKPENLKLMMNLLRDKSPNIQFEAFHVFKVFVASPHKTQPIVEILLKNQPKLIEFLSSFQKERTDDEQFADEKNYLIKQIRDLKKTAP Predict reactive species |
| Full Name | calcium binding protein 39-like |
| Calculated Molecular Weight | 337 aa, 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC010993 |
| Gene Symbol | CAB39L |
| Gene ID (NCBI) | 81617 |
| RRID | AB_2066394 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9S4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAB39L antibody 16137-1-AP | Download protocol |
| IHC protocol for CAB39L antibody 16137-1-AP | Download protocol |
| WB protocol for CAB39L antibody 16137-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CAB39L Polyclonal antibody (Cat# 16137-1-AP) has 1 citation. It appears in Medicina (Kaunas) (2023), a study identifying CAB39L as a diagnostic and prognostic biomarker of kidney renal clear cell carcinoma. The research field is oncology/cancer biomarker discovery, specifically renal cell carcinoma. The antibody was used for IHC in human samples.
| Species | Application | Title |
|---|---|---|
Medicina (Kaunas) Bioinformatics Prediction and Experimental Verification Identify CAB39L as a Diagnostic and Prognostic Biomarker of Kidney Renal Clear Cell Carcinoma |
Reviews
What customers say
The single user review for this product is a 5-star rating from July 2019. The reviewer used it at a 1:1000 dilution for Western Blot on 293T and U2OS cells and reported that the target protein appeared at the expected molecular weight with clean results and no additional background bands in the size range. Overall, the feedback is highly positive, describing the antibody's performance as excellent for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
anwesh (Verified Customer) (07-29-2019) | The protein was found at the right size. No additional background bands were observed around the size range. AWESOME!
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