ETV4 Polyclonal antibody

• Featured Product
• KD/KO Validated

ETV4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 10684-1-AP

Synonyms

PEA3, ETS translocation variant 4, E1A-F, E1AF, Adenovirus E1A enhancer-binding protein

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Validation Data Gallery

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  WB analysis of mouse heart using 10684-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10684-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10684-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 10684-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10684-1-AP (ETV4 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Product Information

10684-1-AP targets ETV4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETV4 fusion protein Ag0984
• Full Name: ets variant 4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 61-65 kDa
• GenBank Accession Number: BC007242
• Gene Symbol: ETV4
• Gene ID (NCBI): 2118
• RRID: AB_2100984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.

Publications

Species	Application	Title
human	WB	Cell; Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription.; Authors - Jaehong Suh
mouse	WB	Dev Cell; ATXN1 protein family and CIC regulate extracellular matrix remodeling and lung alveolarization.; Authors - Lee Yoontae Y ; KD Validated
mouse	WB	Nat Commun; Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.; Authors - Sungjun Park
human	WB	Cancer Res; Targeting pan-ETS factors inhibits melanoma progression.; Authors - Lee Huang
human,mouse	WB	Proc Natl Acad Sci U S A; Erk1/2 inactivation promotes a rapid redistribution of COP1 and degradation of COP1 substrates.; Authors - Weiming Ouyang
human	WB	Oncogene; HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer; Authors - Bei Liu ; KD Validated