CDC37 Monoclonal antibody

CDC37 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C2A6

Cat no : 66420-1-Ig

Synonyms

CDC37, CDC37A, Hsp90 co chaperone Cdc37, P50CDC37

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Validation Data Gallery

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  WB analysis using 66420-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66420-1-Ig (CDC37 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66420-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66420-1-Ig(CDC37 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, ROS1728 cells, RAW 264.7 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66420-1-Ig targets CDC37 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC37 fusion protein Ag0267
• Full Name: cell division cycle 37 homolog (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC000083
• Gene Symbol: CDC37
• Gene ID (NCBI): 11140
• RRID: AB_2881792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC(cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.

Publications

Species	Application	Title
human	IF	Acta Pharmacol Sin; Okicamelliaside targets the N-terminal chaperone pocket of HSP90 disrupts the chaperone protein interaction of HSP90-CDC37 and exerts antitumor activity.; Authors - Chuan-Jing Cheng