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Anticorps Polyclonal de lapin anti-STAT5A-Specific

STAT5A-Specific Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 51074-2-AP

Synonymes

MGF, STAT5, STAT5A, STAT5A-Specific

Galerie de données de validation

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  • Western Blot (WB) analysis of NIH/3T3 cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of NIH/3T3 using 51074-2-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of HeLa using 51074-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    WB analysis of HepG2 using 51074-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 51074-2-AP (STAT5A-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human cervical cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human cervical cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51074-2-AP (STAT5A-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human breast cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51074-2-AP (STAT5A-specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human breast cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51074-2-AP (STAT5A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human lung cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51074-2-AP (STAT5A-specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STAT5A-Specific Polyclonal antibody (51074-2-AP)

    IHC staining of human lung cancer using 51074-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51074-2-AP (STAT5A Antibody) at dilution of 1:50 (under 40x lens).

Applications testées

Résultats positifs en WBcellules NIH/3T3, cellules HeLa, cellules HepG2
Résultats positifs en IHCtissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain, tissu de cancer du sein humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochimie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

51074-2-AP cible STAT5A-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet signal transducer and activator of transcription 5A
Masse moléculaire calculée 794 aa, 92 kDa
Poids moléculaire observé 92 kDa
Numéro d’acquisition GenBankBC027036
Symbole du gène STAT5A
Identification du gène (NCBI) 6776
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GH. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is specific to STAT5A. It has no cross reaction to STAT5B.

Protocole

Product Specific Protocols
WB protocol for STAT5A-Specific antibody 51074-2-APDownload protocol
IHC protocol for STAT5A-Specific antibody 51074-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols