STAT5A/B Polyclonal antibody

STAT5A/B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 12071-1-AP



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MOLT-4 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12071-1-AP targets STAT5A/B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAT5A/B fusion protein Ag2709
Full Name signal transducer and activator of transcription 5B
Calculated Molecular Weight 787 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC020868
Gene Symbol STAT5B
Gene ID (NCBI) 6777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Although this antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT5B, the homology between this segment and the corresponding segment of human STAT5A is 92%. It shows that this antibody can cross-react with STAT5A.


Product Specific Protocols
WB protocol for STAT5A/B antibody 12071-1-APDownload protocol
IHC protocol for STAT5A/B antibody 12071-1-APDownload protocol
IF protocol for STAT5A/B antibody 12071-1-APDownload protocol
IP protocol for STAT5A/B antibody 12071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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