STAT5A/B Polyclonal antibody

STAT5A/B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 12071-1-AP

Synonyms

STAT5B, STAT5

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Validation Data Gallery

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  WB analysis using 12071-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12071-1-AP (STAT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12071-1-AP

  IP result of anti-STAT5A/B (IP:12071-1-AP, 4ug; Detection:12071-1-AP 1:4000) with HeLa cells lysate 1720 ug.

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  IHC staining of human cervical cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12071-1-AP (STAT5A/B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12071-1-AP (STAT5A/B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of K-562 using 12071-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using STAT5A/B antibody (12071-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, MOLT-4 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12071-1-AP targets STAT5A/B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAT5A/B fusion protein Ag2709
• Full Name: signal transducer and activator of transcription 5B
• Calculated Molecular Weight: 787 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC020868
• Gene Symbol: STAT5B
• Gene ID (NCBI): 6777
• RRID: AB_2196933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Although this antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT5B, the homology between this segment and the corresponding segment of human STAT5A is 95%. It shows that this antibody can cross-react with STAT5A.

Publications

Species	Application	Title
rat	WB	Biomed Pharmacother; Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.; Authors - Yu Long
mouse	WB	Br J Pharmacol; HSP90β shapes the fate of Th17 cells with the help of glycolysis-controlled methylation modification; Authors - Ling Yang
rat	WB, IP, IF	Toxicol Appl Pharmacol; PMID: 26493931; Authors - Furong Zhang
rat	WB	Pharm Biol; Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.; Authors - Ying Xu
mouse	WB	Biochem Biophys Res Commun; 1, 25(OH)2D3-induced interaction of vitamin D receptor with p50 subunit of NF-κB suppresses the interaction between KLF5 and p50, contributing to inhibition of LPS-induced macrophage proliferation.; Authors - Dong Ma
rat	WB	Iran J Basic Med Sci; Quercetin of huoxuehuayu tongluo decoction and azithromycin combination therapy effectively improves rat tubal factor infertility by inhibiting inflammation; Authors - Liang Shao