Anticorps Polyclonal de lapin anti-STAT1

• Phare
• Validé par KD/KO

STAT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

137

Avis (3)

N° de cat : 10144-2-AP

Synonymes

Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

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Galerie de données de validation

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  WB analysis using 10144-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10144-2-AP (STAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10144-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10144-2-AP (STAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10144-2-AP

  WB result of STAT1 antibody (10144-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells.

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  IP experiment of PC-3 using 10144-2-AP

  IP result of anti-STAT1 (IP:10144-2-AP, 4ug; Detection:10144-2-AP 1:5000) with PC-3 cells lysate 1575 ug.

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  IHC staining of human breast cancer using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10144-2-AP (STAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10144-2-AP (STAT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10144-2-AP (STAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10144-2-AP (STAT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10144-2-AP (STAT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10144-2-AP (STAT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10144-2-AP

  Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (10144-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of MCF-7 using 10144-2-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human STAT1 (10144-2-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HEK-293, cellules K-562, cellules PC-3
• Résultats positifs en IP: cellules PC-3,
• Résultats positifs en IF/ICC: IFN alpha treated HeLa cells,
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 124 publications below
• IHC: See 4 publications below
• IF: See 14 publications below
• IP: See 8 publications below
• CoIP: See 3 publications below
• ChIP: See 2 publications below

Informations sur le produit

10144-2-AP cible STAT1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STAT1 Protéine recombinante Ag0199
• Nom complet: signal transducer and activator of transcription 1, 91kDa
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 83-87 kDa
• Numéro d’acquisition GenBank: BC002704
• Symbole du gène: STAT1
• Identification du gène (NCBI): 6772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of STAT1? Are there any isoforms of STAT1? Is STAT1 post-translationally modified?

The molecular weight of STAT1 is 91 kDa for full-length STAT1α and 84 kDa for STAT1β, which is devoid of the C-terminal activation domain (PMID: 1502203). Phosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family members and also causes nuclear translocation, while phosphorylation of Ser727 is needed for full activation (PMID: 10851062). STAT1β lacks Ser727 and has therefore been considered to be inactive or acting in a dominant negative manner to STAT1α, although it has been further shown to be capable of transcription activation in vivo (PMID: 24710278).

 What is the subcellular localization of STAT1?

STAT1 is present in the cytoplasm and in the nucleus. Translocation of STAT1 from the cytoplasm to the nucleus is required for its regulation of gene expression and is dependent on phosphorylation (see above). However, low levels of unphosphorylated STAT1, often referred to as U-STAT1, can also be found in the nucleus, where U-STAT1 regulates the stability of heterochromatin by binding to chromatin in monomeric or dimeric states (PMID: 18840523).

 What is the tissue expression pattern of STAT1?

STAT1 is ubiquitously expressed.

Publications

Species	Application	Title
mouse	IF	Cell; Opioid-induced fragile-like regulatory T cells contribute to withdrawal; Authors - Yongsheng Zhu
human,mouse	WB,IF	Nat Commun; ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer; Authors - Shuang Shang
human	WB	Sci Transl Med; TRIB3 reduces CD8+ T cell infiltration and induces immune evasion by repressing the STAT1-CXCL10 axis in colorectal cancer.; Authors - Shuang Shang
mouse	WB	Small; Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.; Authors - Guang-Yu Xu
mouse	WB,IF	Nat Commun; Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone.; Authors - Bin Huang
human	WB	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paul (Verified Customer) (11-24-2022)

Clear band at the expected size range

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: A549 LUAD cells

FH

Alejandro (Verified Customer) (08-22-2022)

Works fine for both WB, with clear bands, and IF, which shows a high specific staining

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/100 for IF and 1/1000 for WB
• Cell Tissue Type: Mouse embrionary fibroblasts

FH

Slavica (Verified Customer) (10-04-2021)

The antibody worked very well!!!

• Applications: Western Blot
• Cell Tissue Type: SHED cell lines

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