STAT4 Polyclonal antibody

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  WB analysis using 51070-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51070-2-AP (STAT4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51070-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51070-2-AP (STAT4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

STAT4 Polyclonal Antibody for WB, ELISA

Cat No. 51070-2-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SLEB11, STAT4

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

51070-2-AP targets STAT4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: signal transducer and activator of transcription 4
• Calculated Molecular Weight: 748 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC031212
• Gene Symbol: STAT4
• Gene ID (NCBI): 6775
• RRID: AB_2286750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14765
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

Publications

Species	Application	Title
mouse	WB	J Immunol; Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells.; Authors - James P Hewitson
human	WB	Food Chem; Cytotoxic withanolides from the stems and leaves of Physalis ixocarpa; Authors - Ke Xiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katie (Verified Customer) (04-08-2022)

Works well for western blot. We have published with this antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary CD4 T cells