Anticorps Polyclonal de lapin anti-SOX2

• Phare
• Validé par KD/KO

SOX2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA, IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

260

Avis (3)

N° de cat : 11064-1-AP

Synonymes

Transcription factor SOX-2, Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3

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Galerie de données de validation

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  WB analysis of HEK-293 using 11064-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11064-1-AP (SOX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11064-1-AP (SOX2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse embryo using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11064-1-AP (SOX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11064-1-AP (SOX2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11064-1-AP (SOX2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11064-1-AP (SOX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11064-1-AP (SOX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11064-1-AP (SOX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11064-1-AP (SOX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11064-1-AP (SOX2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human gliomas using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11064-1-AP (SOX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11064-1-AP (SOX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11064-1-AP (SOX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 11064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11064-1-AP (SOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse embryo using 11064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 11064-1-AP (SOX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 11064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (11064-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human embronic stem cells using 11064-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11064-1-AP at dilution of 1:50. The PE shows staining with 11064-1-AP/PE. The DAPI shows nuclear staining by DAPI.

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  IF Staining of U-251 using 11064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (11064-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of Retinal organoids using 11064-1-AP

  Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for PAX6 with 12323-1-AP at 1:600 (green), and SOX2 with 11064-1-AP at 1:200 (red), Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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  IF Staining of mouse olfactory epithelium tissue using 11064-1-AP

  IF result of SOX2 antibody (11064-1-AP, 1:300) with 1% PLP fixed adult mouse olfactory epithelium.(Red:CK14; Green: SOX2; Blue: DAPI). By Brian Lin, Tufts University.

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  IF Staining of Zebrafish using 11064-1-AP

  IF result of anti-SOX2 (11064-1-AP) whith the midbrain of zebrafish by Dr.Caroline.

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  IF Staining of Zebrafish using 11064-1-AP

  IF result of anti-SOX2 (11064-1-AP) in Zebrafish by Dr. Caroline Parkin.

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  IF Staining of Zebrafish using 11064-1-AP

  IF result of anti-SOX2 (11064-1-AP) with the spine of zebrafish by Dr. Caroline.

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Applications testées

• Résultats positifs en WB: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral de souris, tissu de gliome humain, tissu embryonnaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris, cellules souches embryonnaires humaines, tissu embryonnaire de souris
• Résultats positifs en IF/ICC: cellules U-251
• Résultats positifs en IF: Retinal organoids, tissu de poisson-zèbre, tissu d'épithélium olfactif de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 177 publications below
• IHC: See 47 publications below
• IF: See 76 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11064-1-AP cible SOX2 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, IF et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOX2 Protéine recombinante Ag1530
• Nom complet: SRY (sex determining region Y)-box 2
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34-40 kDa
• Numéro d’acquisition GenBank: BC013923
• Symbole du gène: SOX2
• Identification du gène (NCBI): 6657
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Background

SRY (sex determining region Y)-box 2 is a transcription factor involved in neural stem cell maintenance and has been used in somatic cell reprogramming.

 What is the molecular weight of Sox2?

34 kDa. Sox2 is an intronless gene encoding xx amino acids including HMG (High-mobility group) box domains that can bind to DNA.

 What is the tissue specificity of Sox2?

In embryonic stem cells, Sox2 is highly expressed and is essential in self-renewal. In the developing embryo, Sox2 is found in the neural tube and then in the developing eye and central nervous system (CNS). This expression is highly conserved across organisms (PMID: 25126380; PMID: 8625802).

In adult tissues Sox2 is expressed in neural progenitor cells, which as multipotent cells can both self-renew and differentiate into neurons, astroglia, and oligodendroglia. It maintains their stem cell phenotype and is a useful marker of early neural stem cells (PMID: 16139372).

 What is the role of Sox2 in pluripotency?

Sox2 acts as part of the LIF (Leukemia inhibitory factor) pathway, activated via JAK-STAT signaling to form complexes with other transcription factors such as Oct4 that bind to DNA and work to control pluripotency. Sox2 was described as one of the four original factors that are key to the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) (PMID: 16904174).

 What is the role of Sox2 in cancer?

The ability to control pluripotency means dysregulation of Sox2 can lead to uncontrolled proliferation. In many different cancers, Sox2 has been associated with the metastasis and growth of tumors, mostly due to overexpression (PMID: 28388544). The increased levels of Sox2 RNA and protein in tumor tissue highlight it as a potential therapeutic target (PMID: 25114775).

Publications

Species	Application	Title
human	WB, IF	Cell Stem Cell; The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling.; Authors - Yanying Wang
mouse	IHC	Cell Stem Cell; Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.; Authors - Brian Lin
mouse	IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	IF	Neuron; UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.; Authors - Kejing Zhang
mouse	IF	Mol Psychiatry; Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice.; Authors - C Vicidomini
human	WB,IHC	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

good band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Silvia (Verified Customer) (09-30-2021)

It works well for Immunofluorescence on NPC

• Applications: Immunofluorescence

FH

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval (ph=6)

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina 5dpf

SOX2 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina 5dpf (Cat no:11064-1-AP)

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