NANOG Polyclonal antibody

• Featured Product
• KD/KO Validated

NANOG Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

175

Reviews (3)

Cat no : 14295-1-AP

Synonyms

hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox

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Validation Data Gallery

• 

  WB analysis using 14295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14295-1-AP (NANOG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of human embronic stem cells using 14295-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 14295-1-AP at dilution of 1:200. The PE shows staining with 14295-1-AP/PE. The DAPI shows nuclear staining by DAPI.

• 

  FC experiment of NCCIT using 14295-1-AP

  1X10^6 NCCIT cells were intracellularly stained with 0.2 ug Anti-Human NANOG (14295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: NCCIT cells, rat brain tissue, mouse brain tissue, mouse embryo tissue
• Positive IF detected in: human embronic stem cells
• Positive FC detected in: NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 128 publications below
• IHC: See 14 publications below
• IF: See 50 publications below

Product Information

14295-1-AP targets NANOG in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, marmoset, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NANOG fusion protein Ag5645
• Full Name: Nanog homeobox
• Calculated molecular weight: 35 kDa
• Observed molecular weight: 35-40 kDa
• GenBank accession number: BC160187
• Gene symbol: NANOG
• Gene ID (NCBI): 79923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Publications

Species	Application	Title
mouse	WB	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
mouse		Mol Cell; RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control.; Authors - Xianju Bi
mouse	IF	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
human	WB	Theranostics; Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19.; Authors - Jing Ren
human	WB, IHC	Cancer Res; Loss of SNAIL regulated miR-128-2 on chromosome 3p22.3 targets multiple stem cell factors to promote transformation of mammary epithelial cells.; Authors - Pengxu Qian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fatemeh (Verified Customer) (05-22-2023)

50 ug of protein lysate was used for WB. Blot was blocked with 5% milk and incubated with 1-1000 primary antibody overnight at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1-1000
• Cell Tissue Type: mESCs

NANOG Antibody Western Blot validation (1-1000 dilution) in mESCs (Cat no:14295-1-AP)

FH

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

NANOG Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:14295-1-AP)

FH

Ying (Verified Customer) (08-19-2019)

very good antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mouse primary cardiomyocytes

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