Nanog Polyclonal antibody

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  WB analysis using 14295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14295-1-AP (Nanog antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of human embronic stem cells using 14295-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 14295-1-AP at dilution of 1:200. The PE shows staining with 14295-1-AP/PE. The DAPI shows nuclear staining by DAPI.

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  FC experiment of NICCT using 14295-1-AP

  1X10^6 NCCIT cells were intracellularly stained with 0.4 ug Anti-Human Nanog (14295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

Nanog Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 14295-1-AP

Publications(275)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

hNanog

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Tested Applications

• Positive WB detected in: NCCIT cells, rat brain tissue, mouse brain tissue, mouse embryo tissue
• Positive IF/ICC detected in: human embronic stem cells
• Positive FC (Intra) detected in: NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 186 publications below
• IHC: See 22 publications below
• IF: See 93 publications below

Product Information

14295-1-AP targets Nanog in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, marmoset
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nanog fusion protein Ag5645
• Full Name: Nanog homeobox
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC160187
• Gene Symbol: Nanog
• Gene ID (NCBI): 79923
• RRID: AB_1607719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H9S0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cell Stem Cell; A combined cell and gene therapy approach for homotopic reconstruction of midbrain dopamine pathways using human pluripotent stem cells.; Authors - Niamh Moriarty
human	IF	Cell Stem Cell; Decoding the temporal and regional specification of microglia in the developing human brain.; Authors - Yanxin Li
human	IF	Cell Stem Cell; A cloaked human stem-cell-derived neural graft capable of functional integration and immune evasion in rodent models; Authors - Chiara Pavan
mouse	WB	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
mouse		Mol Cell; RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control.; Authors - Xianju Bi
mouse	IF	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chunxu (Verified Customer) (05-27-2025)

This antibody works very well in WB and IF to determine the stemness either cancer stem cells or mouse embryonic stem cells.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SKOV3, mESCs

FH

Fatemeh (Verified Customer) (05-22-2023)

50 ug of protein lysate was used for WB. Blot was blocked with 5% milk and incubated with 1-1000 primary antibody overnight at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1-1000
• Cell Tissue Type: mESCs

Nanog Antibody Western Blot validation (1-1000 dilution) in mESCs (Cat no:14295-1-AP)

FH

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

Nanog Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:14295-1-AP)

FH

Ying (Verified Customer) (08-19-2019)

very good antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mouse primary cardiomyocytes