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NANOG Polyclonal antibody

NANOG Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 14295-1-AP


hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox

Tested Applications

Positive WB detected inNCCIT cells, rat brain tissue, mouse brain tissue, mouse embryo tissue
Positive IF detected inhuman embronic stem cells
Positive FC detected inNCCIT cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14295-1-AP targets NANOG in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, marmoset, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NANOG fusion protein Ag5645
Full Name Nanog homeobox
Calculated molecular weight 35 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC160187
Gene symbol NANOG
Gene ID (NCBI) 79923
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)


Product Specific Protocols
WB protocol for NANOG antibody 14295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cancer

Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.

Authors - Yang Gu

Mol Cell

RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control.

Authors - Xianju Bi

Nat Commun

Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.

Authors - Buqing Ye

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu


Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19.

Authors - Jing Ren
humanWB, IHC

Cancer Res

Loss of SNAIL regulated miR-128-2 on chromosome 3p22.3 targets multiple stem cell factors to promote transformation of mammary epithelial cells.

Authors - Pengxu Qian


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Fatemeh (Verified Customer) (05-22-2023)

50 ug of protein lysate was used for WB. Blot was blocked with 5% milk and incubated with 1-1000 primary antibody overnight at 4C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1-1000
  • Cell Tissue Type: mESCs
NANOG Antibody Western Blot validation (1-1000 dilution) in mESCs (Cat no:14295-1-AP)

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS Osteosarcoma cells
NANOG Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:14295-1-AP)

Ying (Verified Customer) (08-19-2019)

very good antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: mouse primary cardiomyocytes