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- KD/KO Validated
NANOG Polyclonal antibody
NANOG Polyclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
143
Cat no : 14295-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NCCIT cells, rat brain tissue, mouse brain tissue, mouse embryo tissue |
| Positive IF detected in | human embronic stem cells |
| Positive FC detected in | NCCIT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 113 publications below |
| IHC | See 12 publications below |
| IF | See 33 publications below |
Product Information
14295-1-AP targets NANOG in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, marmoset, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NANOG fusion protein Ag5645 |
| Full Name | Nanog homeobox |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC160187 |
| Gene symbol | NANOG |
| Gene ID (NCBI) | 79923 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NANOG antibody 14295-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. | ||
Mol Cell RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control. | ||
Nat Commun Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice. | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
Theranostics Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19. | ||
Cancer Res Loss of SNAIL regulated miR-128-2 on chromosome 3p22.3 targets multiple stem cell factors to promote transformation of mammary epithelial cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ying (Verified Customer) (08-19-2019) | very good antibody.
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