VSX2 Polyclonal antibody

VSX2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 25825-1-AP

Synonyms

MCOPCB3, MCOP2, HOX10, Homeobox protein CHX10, CHX10

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Validation Data Gallery

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  WB analysis of mouse eye using 25825-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 25825-1-AP (VSX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human retinoblastoma using 25825-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 25825-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 25825-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 25825-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 25825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse eye tissue
• Positive IHC detected in: human retinoblastoma tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

25825-1-AP targets VSX2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VSX2 fusion protein Ag23035
• Full Name: visual system homeobox 2
• Calculated Molecular Weight: 361 aa, 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC128153
• Gene Symbol: VSX2
• Gene ID (NCBI): 338917
• RRID: AB_2880257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cell Stem Cell; Human brain organoids assemble functionally integrated bilateral optic vesicles.; Authors - Elke Gabriel
human	IF	Nat Protoc; Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia; Authors - Elke Gabriel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence