Anticorps Polyclonal de lapin anti-SOD2
SOD2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (5)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugaison
Non conjugué
303
N° de cat : 24127-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules DU 145, cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris |
| Résultats positifs en IP | tissu cérébral de souris, |
| Résultats positifs en IHC | tissu de cancer du foie humain, tissu de cancer du sein humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules HUVEC, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochimie (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 277 publications below |
| IHC | See 27 publications below |
| IF | See 23 publications below |
| IP | See 4 publications below |
| ELISA | See 1 publications below |
| CoIP | See 2 publications below |
Informations sur le produit
24127-1-AP cible SOD2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, bovin, Chèvre, Humain, poisson-zèbre, porc, poulet, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | SOD2 Protéine recombinante Ag21388 |
| Nom complet | superoxide dismutase 2, mitochondrial |
| Masse moléculaire calculée | 25 kDa |
| Poids moléculaire observé | 25 kDa |
| Numéro d’acquisition GenBank | BC016934 |
| Symbole du gène | SOD2 |
| Identification du gène (NCBI) | 6648 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Superoxide dismutase 2 (SOD2), also known as MnSOD (manganese-dependent superoxide dismutase), is a
mitochondrial enzyme that dismutates superoxide ions.
What is the molecular weight of SOD2? Is SOD2 post-translationally modified?
The molecular weight of SOD2 is 25 kDa. SOD2 acts as a homotetramer with manganese ion. SOD2 can be
post-translationally modified, including phosphorylation, nitration, and acetylation. Phosphorylation of SOD2
increases its enzymatic activity and occurs as part of the adaptive response to radiation stress (PMID: 23243068),
while nitration and acetylation decrease SOD2 enzymatic activity (PMID: 20106845, 21172655).
What is the subcellular localization of superoxide dismutases?
SOD2 is the only superoxide dismutase that localizes to the mitochondrial matrix (PMID: 4578091). SOD1 is
predominantly found in the cytoplasm and to a lesser extent in the mitochondrial intermembrane space and
nucleus (PMID: 11507097), while SOD3 localizes extracellularly.
What is the role of SOD2 in neutralizing reactive oxygen species (ROS)?
Superoxide is a main form of ROS that originates directly from the electron transport chain in the mitochondrial
matrix. SOD2 plays a crucial role in mitochondrial ROS homeostasis by catalyzing turnover of superoxide radicals
to hydrogen peroxide and molecular oxygen. ROS are potent physiological signaling molecules but at high doses
they contribute to the pathogenesis of many diseases, including cardiovascular and neurodegenerative diseases
and cancer (PMID: 29669742). SOD2 also associates with mitochondrial DNA (mtDNA), protecting it from
oxidative damage (PMID: 19228881).
What is the tissue expression pattern of SOD2?
SOD2 is ubiquitously expressed.
Can SOD2 be used as a marker of oxidative stress?
SOD2 is a key player in antioxidant defense and contributes to the regulation of oxidative stress. Defects in SOD2
activity or levels can result in oxidative stress. However, SOD2 cannot be used as a marker of oxidative stress
because its expression is not always altered by oxygen stress.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for SOD2 antibody 24127-1-AP | Download protocol |
| IHC protocol for SOD2 antibody 24127-1-AP | Download protocol |
| IF protocol for SOD2 antibody 24127-1-AP | Download protocol |
| IP protocol for SOD2 antibody 24127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA. | ||
Adv Sci (Weinh) IGFBP1 Sustains Cell Survival during Spatially-Confined Migration and Promotes Tumor Metastasis | ||
Nat Commun Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance. | ||
Redox Biol miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation | ||
Nat Commun The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Macarena Lucia (Verified Customer) (10-17-2022) | clear band.
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