Tested Applications
| Positive WB detected in | HEK-293 cells, PC-12 cells, MCF-7 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Neuro-2a cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66428-1-Ig targets FOXO3A in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, macaque |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1289 Product name: Recombinant human FOXO3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 325-673 aa of BC021224 Sequence: SPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG Predict reactive species |
| Full Name | forkhead box O3 |
| Calculated Molecular Weight | 673 aa, 71 kDa |
| Observed Molecular Weight | 85-90 kDa |
| GenBank Accession Number | BC021224 |
| Gene Symbol | FOXO3A |
| Gene ID (NCBI) | 2309 |
| RRID | AB_2881799 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O43524 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FOXO3A antibody 66428-1-Ig | Download protocol |
| IHC protocol for FOXO3A antibody 66428-1-Ig | Download protocol |
| IP protocol for FOXO3A antibody 66428-1-Ig | Download protocol |
| WB protocol for FOXO3A antibody 66428-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Bone Res Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus | ||
Autophagy CDK9 inhibition blocks the initiation of PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis and enhances the therapeutic effects involving mitochondrial dysfunction in hepatocellular carcinoma. | ||
Autophagy The COPS3-FOXO3 positive feedback loop regulates autophagy to promote cisplatin resistance in osteosarcoma
| ||
Autophagy Mitochondrial NAD(+)-mediated mitophagy alleviates type I interferon response to the cytosolic mitochondrial DNA. | ||
Metabolism SEC62 at mitochondria-associated membranes drives MASH progression by suppressing ATAD3B-mediated mitochondrial quality control. |
