Catalase-Specific Polyclonal antibody

Catalase-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 19792-1-AP

Synonyms

catalase, CAT, EC:1.11.1.6

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Validation Data Gallery

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  WB analysis of HT-1080 using 19792-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19792-1-AP (Catalase-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19792-1-AP (Catalase-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19792-1-AP (Catalase-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 19792-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19792-1-AP (Catalase-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 19792-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19792-1-AP (Catalase-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19792-1-AP

  Immunofluorescent analysis of Hela cells, using CAT antibody 19792-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 19792-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAT antibody 19792-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

19792-1-AP targets Catalase-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: catalase
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_001752
• Gene Symbol: Catalase
• Gene ID (NCBI): 847
• RRID: AB_10640433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia. The antibody is specific to CAT.

Publications

Species	Application	Title
mouse	IHC	Proc Natl Acad Sci U S A; Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasis; Authors - Austin C Boese
human	WB	Acta Pharmacol Sin; Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells; Authors - Wa Ding
mouse	WB	Free Radic Biol Med; Menaquinone-4 prevents medication-related osteonecrosis of the jaw through the SIRT1 signaling-mediated inhibition of cellular metabolic stresses-induced osteoblast apoptosis; Authors - Yajun Cui
human	WB	Oxid Med Cell Longev; Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice.; Authors - BuChun Zhang
mouse	WB	Oxid Med Cell Longev; NANOG Attenuates Hair Follicle-Derived Mesenchymal Stem Cell Senescence by Upregulating PBX1 and Activating AKT Signaling.; Authors - Feilin Liu