Anticorps Polyclonal de lapin anti-GLUT3

• Phare
• Validé par KD/KO

GLUT3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

58

Avis (2)

N° de cat : 20403-1-AP

Synonymes

GLUT 3, GLUT3, SLC2A3

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Galerie de données de validation

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  WB analysis using 20403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20403-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 20403-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20403-1-AP (GLUT3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20403-1-AP (GLUT3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Caco-2 using 20403-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using GLUT3 antibody (20403-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Caco-2 using 20403-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using GLUT3 antibody (20403-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HeLa, cellules HepG2, cellules Jurkat, cellules U-251, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cérébral de souris, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 48 publications below
• IHC: See 10 publications below
• IF: See 14 publications below
• IP: See 1 publications below

Informations sur le produit

20403-1-AP cible GLUT3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLUT3 Protéine recombinante Ag14203
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 3
• Masse moléculaire calculée: 496 aa, 54 kDa
• Poids moléculaire observé: 48-60 kDa
• Numéro d’acquisition GenBank: BC039196
• Symbole du gène: GLUT3
• Identification du gène (NCBI): 6515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucose transporter 3 (GLUT3), also known as solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3), is a transporter protein regulating glucose transport across cell membranes and is a primary glucose transporter in neurons.

What is the molecular weight of GLUT3? Is GLUT3 post-translationally modified?

The molecular weight of GLUT3 transporter is 49-52 kDa and depends on the glycosylation pattern, which is tissue-specific (PMID: 9253355 and 9889355). GLUT3 can be N-glycosylated.

What is the subcellular localization of GLUT3?

Glucose transporters, including GLUT3, are multiple-pass integral membrane proteins. GLUT3 is present at the plasma membrane but is also a subject of recycling between the plasma membrane and endosomes. 

What molecules can be transported by GLUT3?

Although the main substrate of GLUT3 transport is glucose, it can also transport mannose, galactose, and xylose.

What is the tissue expression pattern of GLUT3?

GLUT1 and GLUT3 are important for the transport of glucose into the central nervous system. While GLUT1 transports glucose through blood vessels and into astrocytes, GLUT3 is predominantly expressed in neurons, being their main glucose transporter (PMID: 9302083), and in testis (PMID: 18577699).

Publications

Species	Application	Title
mouse	IF	Neuron; Sympathetic nerve-enteroendocrine L cell communication modulates GLP-1 release, brain glucose utilization, and cognitive function; Authors - Wenran Ren
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
human	WB	Cell Death Differ; A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.; Authors - Xiaoyu Qi
human	WB	Autophagy; Newcastle disease virus degrades SIRT3 via PINK1-PRKN-dependent mitophagy to reprogram energy metabolism in infected cells.; Authors - Yabin Gong
mouse	WB	Cell Rep; 16p11.2 haploinsufficiency reduces mitochondrial biogenesis in brain endothelial cells and alters brain metabolism in adult mice; Authors - Alexandria Béland-Millar
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

balawant (Verified Customer) (12-25-2023)

This antibody is very specific and has no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1200
• Cell Tissue Type: Renal Cancer cell line caki-1 and caki-2

FH

Hua (Verified Customer) (02-03-2023)

This antibody works ok. There is one strong non-specific band around 40 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2, mouse liver, and mouse brain tissues

GLUT3 Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, and mouse brain tissues (Cat no:20403-1-AP)

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