LDHA-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

LDHA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

235

Reviews (1)

Cat no : 19987-1-AP

Synonyms

LDHA, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27

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Validation Data Gallery

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  WB analysis using 19987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19987-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19987-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 19987-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19987-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19987-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 19987-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 19987-1-AP

  IP result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human heart using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 19987-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 19987-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 206 publications below
• IHC: See 38 publications below
• IF: See 11 publications below
• IP: See 6 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

19987-1-AP targets LDHA-Specific in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: lactate dehydrogenase A
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 32-37 kDa
• GenBank Accession Number: NM_005566
• Gene Symbol: LDHA
• Gene ID (NCBI): 3939
• RRID: AB_10646429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors -
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
human	WB	Drug Resist Updat; Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression; Authors - Shuai Wang ; KD Validated
human		Cell Stem Cell; Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury; Authors - Shuyong Wang
human		Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christian (Verified Customer) (01-07-2022)

We expected a huge expression in our primary human chondrocytes and it was perfectly and specifically confirmed by this antibody. The antibody worked also for primary rat chondrocytes. (cell lysis with RIPA-lysis buffer, denaturating conditions)

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human primary chondrocytes

LDHA-Specific Antibody Western Blot validation (1:2000 dilution) in human primary chondrocytes (Cat no:19987-1-AP)

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