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  • KD/KO Validated

LDHA-Specific Polyclonal antibody

LDHA-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19987-1-AP

Synonyms

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, LDHA-Specific, PIG19



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19987-1-AP targets LDHA-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lactate dehydrogenase A
Calculated molecular weight 37 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberNM_005566
Gene symbol LDHA
Gene ID (NCBI) 3939
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

Protocols

Product Specific Protocols
WB protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IHC protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IF protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IP protocol for LDHA-Specific antibody 19987-1-APDownload protocol
FC protocol for LDHA-Specific antibody 19987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Authors -
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
human

Nat Immunol

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Authors - Di Huang
humanWB

Cell Metab

Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.

Authors - Ke Wang
mouseWB

Sci Adv

Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice.

Authors - Yuanjiu Lei
mouseWB

Nat Commun

Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.

Authors - Jin-Ho Koh

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christian (Verified Customer) (01-07-2022)

We expected a huge expression in our primary human chondrocytes and it was perfectly and specifically confirmed by this antibody. The antibody worked also for primary rat chondrocytes. (cell lysis with RIPA-lysis buffer, denaturating conditions)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human primary chondrocytes
LDHA-Specific Antibody Western Blot validation (1:2000 dilution) in human primary chondrocytes (Cat no:19987-1-AP)