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- KD/KO Validated
LDHA-Specific Polyclonal antibody
LDHA-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 19987-1-AP
Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
|Positive IP detected in
|mouse kidney tissue
|Positive IHC detected in
|human heart tissue, human skeletal muscle tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
19987-1-AP targets LDHA-Specific in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, goat, chicken
|Host / Isotype
|Rabbit / IgG
|lactate dehydrogenase A
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.
|Product Specific Protocols
|WB protocol for LDHA-Specific antibody 19987-1-AP
|IHC protocol for LDHA-Specific antibody 19987-1-AP
|IF protocol for LDHA-Specific antibody 19987-1-AP
|IP protocol for LDHA-Specific antibody 19987-1-AP
|FC protocol for LDHA-Specific antibody 19987-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christian (Verified Customer) (01-07-2022)
We expected a huge expression in our primary human chondrocytes and it was perfectly and specifically confirmed by this antibody. The antibody worked also for primary rat chondrocytes. (cell lysis with RIPA-lysis buffer, denaturating conditions)