- Featured Product
- KD/KO Validated
LDHA-Specific Polyclonal antibody
LDHA-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 19987-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human heart tissue, human skeletal muscle tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19987-1-AP targets LDHA-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken|
|Host / Isotype||Rabbit / IgG|
|Full Name||lactate dehydrogenase A|
|Calculated molecular weight||37 kDa|
|Observed molecular weight||32-37 kDa|
|GenBank accession number||NM_005566|
|Gene ID (NCBI)||3939|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.
|Product Specific Protocols|
|WB protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|IHC protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|IF protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|IP protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|FC protocol for LDHA-Specific antibody 19987-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nat Cell Biol
RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis
Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.
NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.
Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.
Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice.
Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christian (Verified Customer) (01-07-2022)
We expected a huge expression in our primary human chondrocytes and it was perfectly and specifically confirmed by this antibody. The antibody worked also for primary rat chondrocytes. (cell lysis with RIPA-lysis buffer, denaturating conditions)