Anticorps Polyclonal de lapin anti-Alpha 1 Antitrypsin

Alpha 1 Antitrypsin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 16382-1-AP

Synonymes

a1-antitrypsin, Alpha-1-antitrypsin, Serpin A1, SERPINA1, Alpha 1 antiproteinase

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Galerie de données de validation

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  WB analysis using 16382-1-AP

  human saliva tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16382-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16382-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16382-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 16382-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16382-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human urine using 16382-1-AP

  human urine sample were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16382-1-AP

  IP result of anti-Alpha 1 Antitrypsin (IP:16382-1-AP, 4ug; Detection:16382-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug.

• 

  IHC staining of human liver cancer using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16382-1-PBS

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16382-1-PBS

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16382-1-PBS

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16382-1-PBS

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16382-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha 1 Antitrypsin antibody (16382-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16382-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Alpha 1 Antitrypsin (16382-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu salivaire humain, échantillon d'urine humaine, tissu cutané de souris, tissu hépatique de souris, tissu pulmonaire de souris, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris,
• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du foie humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

16382-1-AP cible Alpha 1 Antitrypsin dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha 1 Antitrypsin Protéine recombinante Ag9369
• Nom complet: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• Masse moléculaire calculée: 418 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC015642
• Symbole du gène: Alpha 1-Antitrypsin
• Identification du gène (NCBI): 5265
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

Publications

Species	Application	Title
human	WB	Cell; Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.; Authors - Chang Cui
human	WB	Nat Commun; Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection.; Authors - Lukas Wettstein
mouse	WB	Nat Commun; The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation.; Authors - Mehrpouya B Mobin
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	IF	Int J Mol Sci; Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 Cells; Authors - Mizuki Muranaka
human	WB	J Nutr Biochem; Characterization of dietary and herbal sourced natural compounds that modulate SEL1L-HRD1 ERAD activity and alleviate protein misfolding in the ER; Authors - Jifeng Yang

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