Alpha Antichymotrypsin Monoclonal antibody

Alpha Antichymotrypsin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

3F2F10

Cat no : 66078-1-Ig

Synonyms

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

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Validation Data Gallery

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  WB analysis of human plasma using 66078-1-Ig

  Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Loading amount：0.7 μL crude plasma was lysised for each lane. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis of human colon using 66078-1-Ig

  Untreated and PNGase F-treated lysates of human colon tissue (25μg/lane) were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  IHC staining of human tonsillitis using 66078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human tonsillitis using 66078-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma, human colon tissue
• Positive IHC detected in: human tonsillitis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 66078-1-Ig is Alpha Antichymotrypsin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha Antichymotrypsin fusion protein Ag2830
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
• Calculated Molecular Weight: 448 aa, 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC010530
• Gene Symbol: Alpha 1 Antichymotrypsin
• Gene ID (NCBI): 12
• RRID: AB_11182502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

Publications

Species	Application	Title
		Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
human	WB,IHC	Breast Cancer; Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells.; Authors - Yingzi Zhang
human	IHC	Pharmaceutics; Distribution of Monocarboxylate Transporters in Brain and Choroid Plexus Epithelium; Authors - Masaki Ueno