PAI-1 Polyclonal antibody

PAI-1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat and More (1)





Cat no : 13801-1-AP


PAI, PAI 1, PAI1, PAI-1, PLANH1, Serpin E1, SERPINE1, PAI-1

Tested Applications

Positive WB detected inHuH-7 cells, HepG2 cells, PC-12 cells, U2OS cells, human placenta tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13801-1-AP targets PAI-1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAI-1 fusion protein Ag4777
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC010860
Gene Symbol PAI-1
Gene ID (NCBI) 5054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).


Product Specific Protocols
WB protocol for PAI-1 antibody 13801-1-APDownload protocol
IHC protocol for PAI-1 antibody 13801-1-APDownload protocol
IF protocol for PAI-1 antibody 13801-1-APDownload protocol
IP protocol for PAI-1 antibody 13801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Nat Commun

A translational program that suppresses metabolism to shield the genome.

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Nat Commun

ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.

Authors - Rokana Taftaf

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A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.

Authors - J J David Ho


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


AIKATERINI (Verified Customer) (03-15-2023)

The antibody was diluted in TBS-T 5% non fat milk.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela
PAI-1 Antibody Western Blot validation (1:1000 dilution) in Hela (Cat no:13801-1-AP)