Validation Data Gallery
|Positive WB detected in
|HuH-7 cells, HepG2 cells, PC-12 cells, U2OS cells, human placenta tissue
|Positive IP detected in
|Positive IHC detected in
|human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13801-1-AP targets PAI-1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat samples.
|Host / Isotype
|Rabbit / IgG
|PAI-1 fusion protein Ag4777
|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
|Calculated molecular weight
|402 aa, 45 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).
|Product Specific Protocols
|WB protocol for PAI-1 antibody 13801-1-AP
|IHC protocol for PAI-1 antibody 13801-1-AP
|IF protocol for PAI-1 antibody 13801-1-AP
|IP protocol for PAI-1 antibody 13801-1-AP
|Click here to view our Standard Protocols
Am J Respir Crit Care Med
CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging
Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer
MiR-18a inhibits BMP4 and HIF-1α Normalizing Brain Arteriovenous Malformations.
A translational program that suppresses metabolism to shield the genome.
ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.
A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
AIKATERINI (Verified Customer) (03-15-2023)
The antibody was diluted in TBS-T 5% non fat milk.