PAI-1 Polyclonal antibody

PAI-1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13801-1-AP

Synonyms

PAI, PAI 1, PAI1, PAI-1, PLANH1, Serpin E1, SERPINE1, PAI-1



Tested Applications

Positive WB detected inHuH-7 cells, HepG2 cells, PC-12 cells, U2OS cells, human placenta tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13801-1-AP targets PAI-1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAI-1 fusion protein Ag4777
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated molecular weight 402 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC010860
Gene symbol SERPINE1
Gene ID (NCBI) 5054
RRIDAB_2186881
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).

Protocols

Product Specific Protocols
WB protocol for PAI-1 antibody 13801-1-APDownload protocol
IHC protocol for PAI-1 antibody 13801-1-APDownload protocol
IF protocol for PAI-1 antibody 13801-1-APDownload protocol
IP protocol for PAI-1 antibody 13801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Respir Crit Care Med

CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging

Authors - Huachun Cui
humanWB

Cancer Discov

Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer

Authors - Nurmaa K Dashzeveg
humanCoIP,WB,IHC

Circ Res

MiR-18a inhibits BMP4 and HIF-1α Normalizing Brain Arteriovenous Malformations.

Authors - Nagore I Marín-Ramos
humanWB,IHC,IF

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff
humanWB

Nat Commun

ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.

Authors - Rokana Taftaf
humanWB

Nat Commun

A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.

Authors - J J David Ho

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

AIKATERINI (Verified Customer) (03-15-2023)

The antibody was diluted in TBS-T 5% non fat milk.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela
PAI-1 Antibody Western Blot validation (1:1000 dilution) in Hela (Cat no:13801-1-AP)