Anticorps Polyclonal de lapin anti-RPGRIP1L

• Phare
• Validé par KD/KO

RPGRIP1L Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 55160-1-AP

Synonymes

CORS3, FTM, JBTS7, KIAA1005, MKS5, Protein fantom, RPGRIP1 like, RPGRIP1 like protein, RPGRIP1L

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Galerie de données de validation

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  WB analysis of HEK-293 using 55160-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55160-1-AP (RPGRIP1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 55160-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 55160-1-AP (RPGRIP1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 55160-1-AP

  IP Result of anti-RPGRIP1L (IP:55160-1-AP, 4ug; Detection:55160-1-AP 1:700) with HEK-293 cells lysate 3200ug.

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  IHC staining of human pancreas using 55160-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55160-1-AP (RPGRIP1L antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 55160-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 55160-1-AP (RPGRIP1L antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 55160-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55160-1-AP (RPGRIP1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 55160-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55160-1-AP (RPGRIP1L antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu pancréatique humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

55160-1-AP cible RPGRIP1L dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RPGRIP1-like
• Masse moléculaire calculée: 151 kDa
• Numéro d’acquisition GenBank: NM_015272
• Symbole du gène: RPGRIP1L
• Identification du gène (NCBI): 23322
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPGRIP1L, also named as FTM, KIAA1005, belongs to the RPGRIP1 family. It negatively regulates signaling through the G-protein coupled thromboxane A2 receptor. RPGRIP1L may be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis.(PMID:17558409) Defects in RPGRIP1L are the cause of Joubert syndrome type 7 (JBTS7). Defects in RPGRIP1L are the cause of Meckel syndrome type 5 (MKS5). This antibody is specific to RPGRIP1L.

Publications

Species	Application	Title
mouse		Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu
		Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu
human		Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human		Am J Hum Genet; Mutations in CSPP1, Encoding a Core Centrosomal Protein, Cause a Range of Ciliopathy Phenotypes in Humans.; Authors - Ranad Shaheen
		J Cell Biol; TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.; Authors - Elle C Roberson
		Dev Cell; Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.; Authors - Quynh P H Nguyen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alice (Verified Customer) (01-25-2019)

Cells fixed in MeOH at -20'C for 5mins.Blocked with 1% w/v Milk/PBSRPGRIP1L antibody diluted in block 1:1000 and co-stained with Acetylated alpha-Tubulin and gamma-tubulin.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hTERT RPE-1

RPGRIP1L Antibody Immunofluorescence, validation (1:1000 dilution) in hTERT RPE-1 (Cat no:55160-1-AP)

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