RPGRIP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPGRIP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13214-1-AP

Synonyms

CORD9, LCA6, RGI1, RGRIP, RPGR interacting protein 1, RPGRIP, RPGRIP1, RPGRIP1d

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Validation Data Gallery

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  WB analysis using 13214-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13214-1-AP (RPGRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13214-1-AP (RPGRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Y79 cells, HEK-293 cells, HL-60 cells
• Positive IHC detected in: mouse eye tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

13214-1-AP targets RPGRIP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPGRIP1 fusion protein Ag4089
• Full Name: retinitis pigmentosa GTPase regulator interacting protein 1
• Calculated Molecular Weight: 147 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC039089
• Gene Symbol: RPGRIP1
• Gene ID (NCBI): 57096
• RRID: AB_10639367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPGRIP1 is a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. The biological functions of RPGRIP1 are complex. Mutations in this gene lead to autosomal recessive congenital blindness. In the eye, it is expressed in amacrine neurons and photoreceptors, and in numerous other tissues, albeit at greatly reduced levels. Moreover, it exists multiple isoforms with species-specific subcellular localization patterns, and the different isoforms perform cell-specific functions. Within photoreceptors, RPGRIP1 localizes predominantly to outer segments (OS) of rods and cones in humans, whereas in bovine eyes, it is found only in rod OS. In contrast, in mice, RPGRIP1 is found primarily in connecting cilia (CC), where it localizes between the ciliary axoneme and the plasma membrane. RPGRIP1 has multiple isoforms with MW 147 kDa, 70-76 kDa, 103 kDa, and 66 kDa.

Publications

Species	Application	Title
mouse	WB,IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering ; KO Validated