TBXA2R Polyclonal antibody

• Featured Product
• KD/KO Validated

TBXA2R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27159-1-AP

Synonyms

Prostanoid TP receptor, TBXA2R, thromboxane A2 receptor, TP, TXA2 R

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Validation Data Gallery

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  WB analysis using 27159-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 27159-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27159-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 27159-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 27159-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells, DU 145 cells, human placenta tissue, MCF-7 cells, PC-3 cells, Hela cells
• Positive IHC detected in: human prostate cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 27159-1-AP is TBXA2R Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBXA2R fusion protein Ag25670
• Full Name: thromboxane A2 receptor
• Calculated Molecular Weight: 343aa,37 kDa; 1043aa,113 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC074750
• Gene Symbol: TBXA2R
• Gene ID (NCBI): 6915
• RRID: AB_2880782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBXA2R, also named as thromboxane A2 receptor, TXA2-R and Prostanoid TP receptor, belongs to G-protein coupled receptor 1 family. Thromboxane A2 receptor is a key molecule in hemostasis as its abnormality leads to bleeding disorders. The TXA2-R receptor is linked via a guanine nucleotide-binding protein (G protein) to phospholipase C (PLC), which hydrolyses phosphoinositides to the two potent stimulatory second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol. IP3 causes increases in cytoplasmic free calcium and diacylglycerol causes activation of protein kinase C. In the kidney, the binding of TXA2-R to glomerular TP receptors causes intense vasoconstriction. The two isoforms expressed in cultured cells show similar ligand binding characteristics and phospholipase C (PLC) activation but oppositely regulate adenylyl cyclase activity (PMID: 8613548).

Publications

Species	Application	Title
mouse	WB	Am J Respir Crit Care Med; Thromboxane-Prostanoid Receptor Signaling Drives Persistent Fibroblast Activation in Pulmonary Fibrosis; Authors - Toshio Suzuki ; KO Validated
rat	WB, IHC	Front Endocrinol (Lausanne); The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats.; Authors - Meihe Li
rat	WB	Eur J Pharmacol; Resveratrol attenuates cyclosporin A-induced upregulation of the thromboxane A2 receptor and hypertension via the AMPK/SIRT1 and MAPK/NF-κB pathways in the rat mesenteric artery; Authors - Qian Li
human,mouse		Arch Dermatol Res; The effect of selinexor on prostaglandin synthesis in virus-positive Merkel cell carcinoma cell lines; Authors - Deepika Narayanan