- Featured Product
- KD/KO Validated
CSPP1 Polyclonal antibody
CSPP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11931-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 7 publications below |
IHC | See 1 publications below |
IF | See 9 publications below |
IP | See 1 publications below |
Product Information
11931-1-AP targets CSPP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CSPP1 fusion protein Ag2521 |
Full Name | centrosome and spindle pole associated protein 1 |
Calculated Molecular Weight | 141 kDa |
Observed Molecular Weight | 141 kDa |
GenBank Accession Number | BC029445 |
Gene Symbol | CSPP1 |
Gene ID (NCBI) | 79848 |
RRID | AB_2087897 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CSPP1 antibody 11931-1-AP | Download protocol |
IF protocol for CSPP1 antibody 11931-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Hum Genet Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy. | ||
Cell Rep A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.
| ||
EMBO Rep CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.
| ||
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
Br J Cancer Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.
|