Anticorps Polyclonal de lapin anti-COX2/ Cyclooxygenase 2/ PTGS2

• Phare
• Validé par KD/KO

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

333

Avis (4)

N° de cat : 12375-1-AP

Synonymes

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

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Galerie de données de validation

• 

  WB analysis using 12375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12375-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12375-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12375-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12375-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX2/ Cyclooxygenase 2/ PTGS2 antibody (12375-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 12375-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using COX2/ Cyclooxygenase 2/ PTGS2 antibody (12375-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules NIH/3T3, cellules Raji, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 300 publications below
• IHC: See 37 publications below
• IF: See 16 publications below

Informations sur le produit

12375-1-AP cible COX2/ Cyclooxygenase 2/ PTGS2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX2/ Cyclooxygenase 2/ PTGS2 Protéine recombinante Ag3025
• Nom complet: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 604 aa, 68 kDa
• Poids moléculaire observé: 70-74 kDa
• Numéro d’acquisition GenBank: BC013734
• Symbole du gène: COX2/PTGS2
• Identification du gène (NCBI): 5743
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.

1. What is the molecular weight of COX2?

The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also 

expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented 

PTGS2 has also previously been detected in AD brains (PMID:14724276).

2. Is COX2 post-translationally modified?

COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and 

s-nitrosylation (PMID: 28939645).

3. What is the difference between COX1, COX2, and COX3?

There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1 

is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly 

induced by various stimuli (as part of infection and inflammatory response) and is controlled by the 

transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered 

non-functional in humans. 

4. I cannot detect COX2 in my sample during western blotting.

Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines, 

and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally, 

the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels 

(PMID: 10966456), which should be taken into account during experimental design.

5. What is the role of COX2 in cancer?

COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with 

greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.

6. What is subcellular localization of COX2?

Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330), 

but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).

Publications

Species	Application	Title
mouse	IF	Adv Sci (Weinh); 3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure; Authors - Jiabin Zhang
human	IHC	Bone Res; Piezo1 expression in chondrocytes controls endochondral ossification and osteoarthritis development; Authors - Laura J Brylka
mouse	IHC	Nat Commun; BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency; Authors - Feixia Wang
human	WB	EBioMedicine; Clearance of apoptotic cells by mesenchymal stem cells contributes to immunosuppression via PGE2.; Authors - Zhuoya Zhang
human	WB	EBioMedicine; The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.; Authors - Pingbo Chen
human	WB	EBioMedicine; Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.; Authors - Ruiqiong Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Uthra (Verified Customer) (10-04-2022)

Paraffin-IHC from embedded mouse skin

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: Skin

COX2/ Cyclooxygenase 2/ PTGS2 Antibody Immunohistochemistry validation (1 to 200 dilution) in Skin (Cat no:12375-1-AP)

FH

Reema (Verified Customer) (09-07-2022)

The antibody works perfectly with WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: rat

FH

Lena (Verified Customer) (02-21-2022)

Used for Paraffin-IHC from embedded mouse bones

• Applications: Immunohistochemistry
• Cell Tissue Type: IHC-P

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA and no further antigen retrieval was necessary. Co-localisation in microglia shown with a known marker (magenta).

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (microglia) at 3dpf

COX2/ Cyclooxygenase 2/ PTGS2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (microglia) at 3dpf (Cat no:12375-1-AP)

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