PLA2G1B Monoclonal antibody

PLA2G1B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66397-1-Ig
Clone No.1F2C10

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, ELISA

Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inpig pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66397-1-Ig targets PLA2G1B in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PLA2G1B fusion protein Ag8812 Predict reactive species
Full Name phospholipase A2, group IB (pancreas)
Calculated Molecular Weight70aa,8 kDa; 148aa,16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC005386
Gene Symbol PLA2G1B
Gene ID (NCBI) 5319
RRIDAB_2881771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04054
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.

Protocols

Product Specific Protocols
WB protocol for PLA2G1B antibody 66397-1-IgDownload protocol
IHC protocol for PLA2G1B antibody 66397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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