Anticorps Polyclonal de lapin anti-PSAP

• Phare
• Validé par KD/KO

PSAP Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (2)

N° de cat : 10801-1-AP

Synonymes

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

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Galerie de données de validation

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  WB analysis using 10801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10801-1-AP (PSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10801-1-AP

  WB result of PSAP antibody (10801-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

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  IHC staining of human breast cancer using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 10801-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10801-1-AP (PSAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 10801-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PSAP antibody (10801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10801-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (10801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 10801-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10801-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cervelet de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HEK-293, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• ELISA: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

10801-1-AP cible PSAP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Chèvre, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSAP Protéine recombinante Ag1194
• Nom complet: prosaposin
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC001503
• Symbole du gène: PSAP
• Identification du gène (NCBI): 5660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.

Publications

Species	Application	Title
human	IF,WB	Nat Neurosci; Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis.; Authors - Ruilin Tian
human	IF	Adv Sci (Weinh); Integrative Transcriptomic Analyses of Hippocampal-Entorhinal System Subfields Identify Key Regulators in Alzheimer's Disease; Authors - Dan Luo
human	WB	Nat Commun; Prosaposin is a regulator of progranulin levels and oligomerization.; Authors - Alexandra M Nicholson ; KD Validated
human	WB	Nat Commun; Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.; Authors - Xiaolai Zhou
mouse	WB	Mol Neurodegener; Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains.; Authors - Md Golam Sharoar
mouse	WB	Proc Natl Acad Sci U S A; Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.; Authors - Constance S Petit

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sharan (Verified Customer) (05-28-2020)

The antibody works well in western blot assays with minimum cross-reactivity

• Applications: Western Blot

PSAP Antibody Western Blot validation ( dilution) in (Cat no:10801-1-AP)

FH

Krishnendu (Verified Customer) (02-27-2020)

Very specific for AIMP3

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 for WB, 1:100 For IP and 1:250 for IF
• Cell Tissue Type: HEK293T, MCF7

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