GM2A Polyclonal antibody

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  WB analysis of rat kidney using 10864-2-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10864-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10864-2-AP

  IP result of anti-GM2A (IP:10864-2-AP, 3ug; Detection:10864-2-AP 1:600) with HEK-293 cells lysate 2800ug.

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  IHC staining of human placenta using 10864-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10864-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HEK-293 using 10864-2-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (10864-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

GM2A Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 10864-2-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

Sphingolipid activator protein 3, SAP-3, SAP3, GM2-AP, GM2AP

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Tested Applications

• Positive WB detected in: rat kidney tissue, mouse kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

10864-2-AP targets GM2A in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1295

  Product name: Recombinant human GM2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-193 aa of BC009273

  Sequence: MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

• Full Name: GM2 ganglioside activator
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009273
• Gene Symbol: GM2A
• Gene ID (NCBI): 2760
• RRID: AB_2110807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17900
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Basic Res Cardiol; Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy.; Authors - Olga Eleftheriadou
human	WB	Cell Struct Funct; Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells.; Authors - Seiichiro Ishihara ; KD Validated
human	WB	Mol Neurodegener; Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity; Authors - Yi-Chen Hsieh
human	WB	Nat Commun; Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis; Authors - Sebastian Boland