|Positive WB detected in||rat kidney tissue, mouse kidney tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10864-2-AP targets GM2A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GM2A fusion protein Ag1295|
|Full Name||GM2 ganglioside activator|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC009273|
|Gene ID (NCBI)||2760|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.
Basic Res Cardiol
Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy.
Cell Struct Funct
Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells.
Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity
Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis