Cathepsin D Polyclonal antibody

Cathepsin D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 55021-1-AP

Synonyms

Cathepsin D, CLN10, CPSD, CTSD

---

Validation Data Gallery

• 

  WB analysis using 55021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55021-1-AP (Cathepsin D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 55021-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55021-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 55021-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55021-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 2 publications below

Product Information

55021-1-AP targets Cathepsin D in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cathepsin D
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_001909
• Gene Symbol: Cathepsin D
• Gene ID (NCBI): 1509
• RRID: AB_10863128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)

Publications

Species	Application	Title
mouse	IF	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	Toxicology; 1,3-dichloro-2-propanol induced lipid accumulation by blocking autophagy flux in HepG2 cells.; Authors - Jing Lu
human	IF	Pathogens; Sputum from People with Cystic Fibrosis Reduces the Killing of Methicillin-Resistant Staphylococcus aureus by Neutrophils and Diminishes Phagosomal Production of Reactive Oxygen Species; Authors - Kayla M Fantone
human	WB	Cell Death Discov; A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein.; Authors - Pengyun Li
human	WB	Chem Biol Interact; In addition to its endosomal escape effect, platycodin D also synergizes with ribosomal inactivation protein to induce apoptosis in hepatoma cells through AKT and MAPK signaling pathways.; Authors - Jin Lei
human	WB	Biochem Biophys Res Commun; Bifendate inhibits autophagy at multiple steps and attenuates oleic acid-induced lipid accumulation; Authors - Weigang Yuan