Anticorps Polyclonal de lapin anti-PKC Gamma

PKC Gamma Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 14364-1-AP

Synonymes

PRKCG, Protein kinase C gamma type, PKC-gamma, PKCG, PKCC

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Galerie de données de validation

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  WB analysis of mouse brain using 14364-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14364-1-AP (PKC gamma antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14364-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14364-1-AP (PKC gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14364-1-AP

  IP result of anti-PKC Gamma (IP:14364-1-AP, 4ug; Detection:14364-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human brain using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14364-1-AP (PKC gamma antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14364-1-AP (PKC gamma antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cerebellum using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14364-1-AP (PKC gamma antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14364-1-AP (PKC gamma antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 14364-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 14364-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14364-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PKC Gamma (14364-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de rat, tissu de cervelet humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules SH-SY5Y
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

14364-1-AP cible PKC Gamma dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKC Gamma Protéine recombinante Ag5681
• Nom complet: protein kinase C, gamma
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 76-80 kDa
• Numéro d’acquisition GenBank: BC047876
• Symbole du gène: PKC Gamma
• Identification du gène (NCBI): 5582
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.

Publications

Species	Application	Title
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
rat	WB	Chemosphere; Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.; Authors - Mingdan You
human	WB	Cell Oncol (Dordr); The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells; Authors - Yao-Qi Zhou
mouse	IHC	J Neurosci; Dendritic Spine Dynamics after Peripheral Nerve Injury: An Intravital Structural Study.; Authors - Curtis A Benson
rat	WB	J Ethnopharmacol; Analgesic effects and possible mechanisms of iridoid glycosides from Lamiophlomis rotata (Benth.) Kudo in rats with spared nerve injury.; Authors - Yanan Zheng
mouse	WB,IF	Inflamm Bowel Dis; LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin.; Authors - Xiaomin Su

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chiara (Verified Customer) (10-04-2021)

Clear single band

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mesenchymal Stem cell

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