PKC Alpha Polyclonal antibody

• Featured Product
• KD/KO Validated

PKC Alpha Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

52

Reviews (4)

Cat no : 21991-1-AP

Synonyms

PRKCA, PKCα, PKC-A, PKCA, PKC A

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Validation Data Gallery

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  WB analysis using 21991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC Alpha antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21991-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21991-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 21991-1-AP

  IP result of anti-PKC Alpha (IP:21991-1-AP, 4ug; Detection:21991-1-AP 1:3000) with NIH/3T3 cells lysate 1320 ug.

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  IHC staining of human thyroid cancer using 21991-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid cancer using 21991-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 21991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21991-1-AP (PKC Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21991-1-AP (PKC Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21991-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 21991-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 21991-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 21991-1-AP (PKC alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21991-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (21991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Jurkat using 21991-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PKC Alpha Polyclonal antibody (21991-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, human peripheral blood platelets, rat kidney tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human thyroid cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, NIH/3T3 cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 42 publications below
• IHC: See 3 publications below
• IF: See 12 publications below

Product Information

21991-1-AP targets PKC Alpha in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKC Alpha fusion protein Ag17275
• Full Name: protein kinase C, alpha
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: AK055431
• Gene Symbol: PKC Alpha
• Gene ID (NCBI): 5578
• RRID: AB_2878965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Publications

Species	Application	Title
human	WB	Theranostics; MED13L integrates Mediator-regulated epigenetic control into lung cancer radiosensitivity.; Authors - Nasha Zhang
human	IF	Cell Death Dis; ATF4/CEMIP/PKCα promotes anoikis resistance by enhancing protective autophagy in prostate cancer cells.; Authors - Ying Yu
human	WB	Cell Death Differ; β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.; Authors - Jia Ma
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	Oncogenesis; Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth; Authors - Chunming Gu
human	WB	Front Pharmacol; Cellular mechanism of action of forsythiaside for the treatment of diabetic kidney disease; Authors - Chunmei Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (11-07-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence

FH

Chiara (Verified Customer) (10-04-2021)

Works very well in Immunoblot

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mesenchymal Stem cell

FH

Samantha (Verified Customer) (02-24-2020)

Bovine vascular smooth muscle cells were transfected with either control siRNA or PKCalpha siRNA for up to 7 days, with transfections repeated every 48-72 hours. Blocking agent: 5% (w/v) milk in TBST for 1hrPrimary antibody diluted in 5% (w/v) milk in TBST; incubated overnight at 4 degrees.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:750
• Cell Tissue Type: Bovine vascular smooth muscle cells

PKC Alpha Antibody Western Blot, validation (1:750 dilution) in Bovine vascular smooth muscle cells (Cat no:21991-1-AP)

FH

Ryan (Verified Customer) (01-10-2018)

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish Retina

PKC Alpha Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:21991-1-AP)