PKC Epsilon Polyclonal antibody

• Featured Product
• KD/KO Validated

PKC Epsilon Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 20877-1-AP

Synonyms

PRKCE, Protein kinase C epsilon type, PKCE, nPKC-epsilon, EC:2.7.11.13

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Validation Data Gallery

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  WB analysis using 20877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC Epsilon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20877-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC epsilon antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 20877-1-AP

  IP result of anti-PKC Epsilon (IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:800) with SH-SY5Y cells lysate 1240 ug.

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  IP experiment of SH-SY5Y using 20877-1-AP

  IP result of anti-PKC Epsilon (IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:20000) with SH-SY5Y cells lysate 1240 ug.

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  IHC staining of mouse brain using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse brain using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human thyroid cancer using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20877-1-AP (PKC epsilon antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human thyroid cancer using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20877-1-AP (PKC epsilon antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse brain using 20877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 20877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 20877-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 20877-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U2OS using 20877-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 20877-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PKC Epsilon (20877-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells, rat brain tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells, U2OS cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

20877-1-AP targets PKC Epsilon in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKC Epsilon fusion protein Ag14976
• Full Name: protein kinase C, epsilon
• Calculated Molecular Weight: 737 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC109033
• Gene Symbol: PKC Epsilon
• Gene ID (NCBI): 5581
• RRID: AB_10697812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKC Epsilon (Protein kinase C epsilon type) is also named as PKC-ε, PRKCE and PKCE. It belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PKC-ε, one of the novel isoforms, as a critical player in membrane mobilization during phagocytosis (PMID: 9069266). KC-ε is tethered to the Golgi through binding of its pseudosubstrate domain to phosphatidylinositol-4-phosphate (PI4P) (PMID: 28539432). PKC-ε traffics on microtubule-associated vesicles from the Golgi to the forming phagosome. As the majority of macrophage PKC-ε is cytosolic, and PKCs translocate from the cytosol to their sites of activity, it predicted that cytosolic PKC-ε concentrated at phagosomes to facilitate membrane addition (PMID: 34622926). Two PKC isozymes of the novel group, PKCε and PKCδ, have different and sometimes opposite effects. PKCε stimulates cell growth and differentiation while PKCδ is apoptotic (PMID: 21810427).

Publications

Species	Application	Title
mouse	IF	Elife; cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning; Authors - Xin-Tai Wang
mouse	WB	Biochem Pharmacol; Thromboxane A2-TP axis promotes adipose tissue macrophages M1 polarization leading to insulin resistance in obesity; Authors - Ruijie Xu
mouse	WB	J Cell Mol Med; Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid-induced biochemical alteration in mice.; Authors - Peng Liu
	WB	Fish Shellfish Immunol; In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.; Authors - Zhong-Wen Chang
human	WB	Mol Biol Cell; MiR-34a regulates blood-tumor barrier function by targeting protein kinase Cɛ.; Authors - Wei Zhao
human	WB	Oxid Med Cell Longev; p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.; Authors - Ming Zhang