PKC Zeta Polyclonal antibody

• Featured Product
• KD/KO Validated

PKC Zeta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10860-1-AP

Synonyms

PRKCZ, Protein kinase C zeta type, PKC2, nPKC-zeta, EC:2.7.11.13

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Validation Data Gallery

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  WB analysis of HEK-293 using 10860-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10860-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10860-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10860-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of NIH/3T3 using 10860-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PKC Zeta antibody (10860-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10860-1-AP targets PKC Zeta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKC Zeta fusion protein Ag1310
• Full Name: protein kinase C, zeta
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC014270
• Gene Symbol: PKC Zeta
• Gene ID (NCBI): 5590
• RRID: AB_2300348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Sci Rep; aPKCζ-dependent Repression of Yap is Necessary for Functional Restoration of Irradiated Salivary Glands with IGF-1.; Authors - Alejandro M Chibly ; KO Validated
human,mouse	WB,IHC	Cancers (Basel); Hypermethylation of PRKCZ Regulated by E6 Inhibits Invasion and EMT via Cdc42 in HPV-Related Head and Neck Squamous Cell Carcinoma; Authors - Hao-Fan Wang ; KD Validated