Anticorps Monoclonal anti-PGC1a

• Phare
• Validé par KD/KO

PGC1a Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

291

CloneNo.

1C1B2

Avis (5)

N° de cat : 66369-1-Ig

Synonymes

LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PGC-1α, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A

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Galerie de données de validation

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  WB analysis using 66369-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66369-1-Ig

  WB result of PGC1a antibody (66369-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and PGC1a knockout HeLa cells.

• 

  WB analysis using 66369-1-Ig

  Western blot analysis of PPARGC1A in various cell lines and tissues using Proteintech antibody 66369-1-Ig at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66369-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 66369-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66369-1-Ig(PPARGC1A antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de porc, cellules C2C12, cellules HSC-T6, cellules ROS1728, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 274 publications below
• IHC: See 19 publications below
• IF: See 32 publications below
• IP: See 5 publications below
• ELISA: See 1 publications below

Informations sur le produit

66369-1-Ig cible PGC1a dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, porc, poulet, souris, Hamster
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 70 kDa, 100 kDa
• Numéro d’acquisition GenBank: NM_013261
• Symbole du gène: PPARGC1A
• Identification du gène (NCBI): 10891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

Publications

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
mouse	WB	Sci Adv; Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.; Authors - Jun Seok Son
chicken	WB,IF	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
	WB	J Autoimmun; Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis; Authors - Jean-Baptiste Vulsteke
mouse	WB	Nat Commun; Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.; Authors - Yan-Ting Chen
mouse	WB	Nat Commun; METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice.; Authors - Yuqin Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Michaela (Verified Customer) (11-24-2023)

Worked well in 15μg mouse brain tissue lysate in Western Blot. Band appears between 60-100 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse brain tissue

FH

No (Verified Customer) (10-24-2023)

A good antibody to detect endogenous PGC1a.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

PGC1a Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66369-1-Ig)

FH

Mi (Verified Customer) (03-07-2023)

It doesn´t work well in human white and brown adipocyte cells, we got bands at unexpected size. Further KO validation may be needed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

FH

Huai-Chin (Verified Customer) (07-11-2022)

There are several non-specific bands. At least there's a dominant band after adipogenesis and we can use this antibody at a fairly high dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: 3T3-L1 cells

FH

Mohammad (Verified Customer) (01-21-2020)

It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.

• Applications: Western Blot,
• Cell Tissue Type: Mouse hippocampus

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