Anticorps Polyclonal de lapin anti-PPARA

• Phare
• Validé par KD/KO

PPARA Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IP, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

179

N° de cat : 15540-1-AP

Synonymes

hPPAR, NR1C1, PPAR, PPAR alpha, PPARA, PPARα

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Galerie de données de validation

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  WB analysis using 15540-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 15540-1-AP (PPARA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 15540-1-AP

  IP result of anti-PPARA (IP:15540-1-AP, 4ug; Detection:15540-1-AP 1:300) with U-937 cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules C2C12,
• Résultats positifs en IP: cellules U-937,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 169 publications below
• IF: See 13 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

15540-1-AP cible PPARA dans les applications de WB, IP, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, porc, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPARA Protéine recombinante Ag7896
• Nom complet: peroxisome proliferator-activated receptor alpha
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC000052
• Symbole du gène: PPARA
• Identification du gène (NCBI): 5465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling; Authors - Jing Zhong
human	WB	Sci Adv; Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.; Authors - Leiming Wang
mouse	WB	Hepatology; The histone methyltransferase Suv39h2 contributes to nonalcoholic steatohepatitis in mice.; Authors - Zhiwen Fan
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
human	WB	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
mouse	WB	Nat Commun; Hepatic HuR modulates lipid homeostasis in response to high-fat diet.; Authors - Zhuojun Zhang

Produits associés

PPAR Gamma Polyclonal antibody

16643-1-AP

PPARD Polyclonal antibody

10156-2-AP

PGC1a Polyclonal antibody

20658-1-AP

RXRA Polyclonal antibody

21218-1-AP

NR1H3 Polyclonal antibody

14351-1-AP

ER Polyclonal antibody

21244-1-AP