Anticorps Polyclonal de lapin anti-uPAR

uPAR Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 10286-1-AP

Synonymes

CD87, MO3, PLAUR, U PAR, UPAR, URKR

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Galerie de données de validation

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  WB analysis using 10286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 10286-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules A2780, cellules U-251, cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

10286-1-AP cible uPAR dans les applications de WB, IHC, CoIP, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: uPAR Protéine recombinante Ag0187
• Nom complet: PLAUR
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35-70 kDa
• Numéro d’acquisition GenBank: BC002788
• Symbole du gène: uPAR
• Identification du gène (NCBI): 5329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

Publications

Species	Application	Title
mouse	IF	Mol Cell; A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation; Authors - Xuehui Lyu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
mouse	IHC	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
human	WB	Nucleic Acids Res; Regulation of u-PAR gene expression by H2A.Z is modulated by the MEK-ERK/AP-1 pathway.; Authors - Santosh Chauhan
human,mouse	WB,IHC	FASEB J; CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.; Authors - Zeyu Su
human	IHC	J Inflamm Res; PLAUR as a Potential Biomarker Associated with Immune Infiltration in Bladder Urothelial Carcinoma.; Authors - Mulin Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ruchi (Verified Customer) (07-26-2022)

IF on mouse skin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse skin

uPAR Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:10286-1-AP)

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