Anticorps Polyclonal de lapin anti-PINK1

• Phare
• Validé par KD/KO

PINK1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

272

Avis (5)

N° de cat : 23274-1-AP

Synonymes

BRPK, PARK6, PINK1, PTEN induced putative kinase 1

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Galerie de données de validation

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  WB analysis of HeLa using 23274-1-AP

  WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells.

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  WB analysis of HeLa using 23274-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23274-1-AP

  Non-treated and CCCP treated HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23274-1-AP (PINK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules PC-12
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 17 publications below
• WB: See 252 publications below
• IHC: See 23 publications below
• IF: See 49 publications below
• IP: See 2 publications below
• CoIP: See 4 publications below

Informations sur le produit

23274-1-AP cible PINK1 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, poisson-zèbre, porc, singe, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PINK1 Protéine recombinante Ag19825
• Nom complet: PTEN induced putative kinase 1
• Masse moléculaire calculée: 581 aa, 63 kDa
• Poids moléculaire observé: 65 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC028215
• Symbole du gène: PINK1
• Identification du gène (NCBI): 65018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Publications

Species	Application	Title
human	WB	Nat Commun; Gonococcal OMV-delivered PorB induces epithelial cell mitophagy; Authors - Shuai Gao
human	WB	Mol Cell; The HRI branch of the integrated stress response selectively triggers mitophagy; Authors - Yogaditya Chakrabarty
human,mouse	WB,IP	J Hazard Mater; Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1; Authors - Hao Chen
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu
mouse	WB	Redox Biol; Atox1 protects hippocampal neurons after traumatic brain injury via DJ-1 mediated anti-oxidative stress and mitophagy; Authors - Pengzhan Zhao
human	WB	Nucleic Acids Res; DNA damage invokes mitophagy through a pathway involving Spata18.; Authors - Xiuli Dan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

aidan (Verified Customer) (04-10-2024)

Good results. Would try higher dilution next time (1:500) on precast gel. Used handcast gel overnight incubation at 4 degrees. Blocked 1 hr 5% milk

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Muller retinal mouse

PINK1 Antibody Western Blot validation (1:1000 dilution) in Muller retinal mouse (Cat no:23274-1-AP)

FH

Nin (Verified Customer) (02-27-2023)

It is okay to probe PINK1 with two bands although there are some weak non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

PINK1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:23274-1-AP)

FH

Yahir Alberto (Verified Customer) (11-12-2021)

The antibody works fine with overnight incubation at 4 °C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T cells

PINK1 Antibody Western Blot validation (1:1000 dilution) in HEK 293T cells (Cat no:23274-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

HONGXUE (Verified Customer) (08-19-2019)

The specific is not good. You can detect many bands using WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 lysate

PINK1 Antibody Western Blot, validation (1:1000 dilution) in HepG2 lysate (Cat no:23274-1-AP)

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