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  • KD/KO Validated

PARK7,DJ-1 Polyclonal antibody

PARK7,DJ-1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11681-1-AP

Synonyms

DJ 1, DJ1, DJ-1, Oncogene DJ1, PARK7, PARK7,DJ-1, Parkinson disease protein 7, Protein DJ 1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human liver cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11681-1-AP targets PARK7,DJ-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARK7,DJ-1 fusion protein Ag2287
Full Name Parkinson disease (autosomal recessive, early onset) 7
Calculated molecular weight 189 aa, 20 kDa
Observed molecular weight 20 kDa, 25 kDa
GenBank accession numberBC008188
Gene symbol PARK7
Gene ID (NCBI) 11315
RRIDAB_2160099
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

Protocols

Product Specific Protocols
WB protocol for PARK7,DJ-1 antibody 11681-1-APDownload protocol
IHC protocol for PARK7,DJ-1 antibody 11681-1-APDownload protocol
IF protocol for PARK7,DJ-1 antibody 11681-1-APDownload protocol
IP protocol for PARK7,DJ-1 antibody 11681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Chemical proteomics reveals new targets of cysteine sulfinic acid reductase.

Authors - Salma Akter
mouseWB

Nat Commun

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

Authors - Zhisheng Xu
  • KD Validated
mouseIF

PLoS Biol

Extracellular DJ-1 induces sterile inflammation in the ischemic brain.

Authors - Koutarou Nakamura
humanWB

Mol Cell Proteomics

Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.

Authors - Rui Sun
human, mouseWB,IF

Mol Cell Biol

ROS-mediated DJ-1 monomerization modulates intracellular trafficking involving Karyopherin β2.

Authors - Benny Björkblom
humanWB

J Cell Mol Med

FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling

Authors - Xiaojing Xing

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

X (Verified Customer) (07-11-2022)

A very good and clean antibody with the right molecular weight for WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
PARK7,DJ-1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:11681-1-AP)
FH

Daniel (Verified Customer) (04-16-2019)

Specific band in RCC4 cells

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human kidney cancer cells (RCC4)
PARK7,DJ-1 Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:11681-1-AP)