Validation Data Gallery
|Positive WB detected in
|HeLa cells, HEK-293 cells, Jurkat cells
|Positive IP detected in
|Positive IHC detected in
|human gliomas tissue, human liver cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:1000-1:4000
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11681-1-AP targets PARK7,DJ-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|PARK7,DJ-1 fusion protein Ag2287
|Parkinson disease (autosomal recessive, early onset) 7
|Calculated molecular weight
|189 aa, 20 kDa
|Observed molecular weight
|20 kDa, 25 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).
|Product Specific Protocols
|WB protocol for PARK7,DJ-1 antibody 11681-1-AP
|IHC protocol for PARK7,DJ-1 antibody 11681-1-AP
|IF protocol for PARK7,DJ-1 antibody 11681-1-AP
|IP protocol for PARK7,DJ-1 antibody 11681-1-AP
|Click here to view our Standard Protocols
Nat Chem Biol
Chemical proteomics reveals new targets of cysteine sulfinic acid reductase.
Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.
Extracellular DJ-1 induces sterile inflammation in the ischemic brain.
Mol Cell Proteomics
Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.
Mol Cell Biol
ROS-mediated DJ-1 monomerization modulates intracellular trafficking involving Karyopherin β2.
J Cell Mol Med
FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
X (Verified Customer) (07-11-2022)
A very good and clean antibody with the right molecular weight for WB.
Daniel (Verified Customer) (04-16-2019)
Specific band in RCC4 cells