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- KD/KO Validated
PARK2/Parkin Polyclonal antibody
PARK2/Parkin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
185
Cat no : 14060-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C6 cells, HEK-293 cells, mouse brain tissue, SH-SY5Y cells, PC-12 cells, mouse liver tissue |
Positive IHC detected in | mouse kidney tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells, mouse brain tissue, mouse heart tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 8 publications below |
WB | See 166 publications below |
IHC | See 17 publications below |
IF | See 43 publications below |
IP | See 2 publications below |
CoIP | See 3 publications below |
Product Information
14060-1-AP targets PARK2/Parkin in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, cattle, ducks, rabbit, pig, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PARK2/Parkin fusion protein Ag5092 |
Full Name | Parkinson disease (autosomal recessive, juvenile) 2, parkin |
Calculated molecular weight | 52 kDa |
Observed molecular weight | 42-52 kDa |
GenBank accession number | BC022014 |
Gene symbol | PARK2 |
Gene ID (NCBI) | 5071 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing with molecular weights of 24, 31, 36 and 42-52 kDa. Sometimes an additional band of 70 kDa or 110 kDa may be detected, which is caused by ubiquitination modification or formation of Parkin complex (PMID: 10976934, PMID: 18190519).
Protocols
Product Specific Protocols | |
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WB protocol for PARK2/Parkin antibody 14060-1-AP | Download protocol |
IHC protocol for PARK2/Parkin antibody 14060-1-AP | Download protocol |
IF protocol for PARK2/Parkin antibody 14060-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota | ||
Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. | ||
Autophagy Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. | ||
Redox Biol Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats | ||
Aging Cell Loss of legumain induces premature senescence and mediates aging-related renal fibrosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ajte (Verified Customer) (03-08-2022) | It works well for me
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FH Elyssa (Verified Customer) (11-11-2019) | This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.
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