PARK2/Parkin Polyclonal antibody

• Featured Product
• KD/KO Validated

PARK2/Parkin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

185

Reviews (2)

Cat no : 14060-1-AP

Synonyms

AR JP, LPRS2, PARK2, PARK2/Parkin, Parkin, Parkinson disease protein 2, PDJ, PRKN

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Validation Data Gallery

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  WB analysis using 14060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14060-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14060-1-AP (PARK2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14060-1-AP (PARK2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 14060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14060-1-AP (PARK2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14060-1-AP (PARK2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 14060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using PARK2/Parkin antibody (14060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 14060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 14060-1-AP (PARK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PARK2/Parkin antibody (14060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PARK2/Parkin antibody (14060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, HEK-293 cells, mouse brain tissue, SH-SY5Y cells, PC-12 cells, mouse liver tissue
• Positive IHC detected in: mouse kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells, mouse brain tissue, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 8 publications below
• WB: See 166 publications below
• IHC: See 17 publications below
• IF: See 43 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Product Information

14060-1-AP targets PARK2/Parkin in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, cattle, ducks, rabbit, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARK2/Parkin fusion protein Ag5092
• Full Name: Parkinson disease (autosomal recessive, juvenile) 2, parkin
• Calculated molecular weight: 52 kDa
• Observed molecular weight: 42-52 kDa
• GenBank accession number: BC022014
• Gene symbol: PARK2
• Gene ID (NCBI): 5071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing with molecular weights of 24, 31, 36 and 42-52 kDa. Sometimes an additional band of 70 kDa or 110 kDa may be detected, which is caused by ubiquitination modification or formation of Parkin complex (PMID: 10976934, PMID: 18190519).

Publications

Species	Application	Title
	WB	Nat Commun; Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota; Authors - Ya Liu
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
rat	IF	Redox Biol; Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats; Authors - Mengyu Li
human	WB	Nucleic Acids Res; DNA damage invokes mitophagy through a pathway involving Spata18.; Authors - Xiuli Dan
mouse	WB	Aging Cell; Loss of legumain induces premature senescence and mediates aging-related renal fibrosis.; Authors - Dekun Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ajte (Verified Customer) (03-08-2022)

It works well for me

• Applications: Western Blot

FH

Elyssa (Verified Customer) (11-11-2019)

This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T47D, MCF7, CAMA1 (only worked in T47D)

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