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PARK2/Parkin Polyclonal antibody
PARK2/Parkin Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 14060-1-AP
Validation Data Gallery
|Positive WB detected in||C6 cells, HEK-293 cells, mouse brain tissue, SH-SY5Y cells, PC-12 cells, mouse liver tissue|
|Positive IHC detected in||mouse kidney tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, mouse brain tissue, mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14060-1-AP targets PARK2/Parkin in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, cattle, ducks, rabbit, pig, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PARK2/Parkin fusion protein Ag5092|
|Full Name||Parkinson disease (autosomal recessive, juvenile) 2, parkin|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||42-52 kDa|
|GenBank accession number||BC022014|
|Gene ID (NCBI)||5071|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing with molecular weights of 24, 31, 36 and 42-52 kDa. Sometimes an additional band of 70 kDa or 110 kDa may be detected, which is caused by ubiquitination modification or formation of Parkin complex (PMID: 10976934, PMID: 18190519).
Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota
Acta Pharm Sin B
Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.
Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.
Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats
Nucleic Acids Res
DNA damage invokes mitophagy through a pathway involving Spata18.
Loss of legumain induces premature senescence and mediates aging-related renal fibrosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ajte (Verified Customer) (03-08-2022)
It works well for me
Elyssa (Verified Customer) (11-11-2019)
This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.