Tim23 Polyclonal antibody

• Featured Product
• KD/KO Validated

Tim23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

137

Cat no : 11123-1-AP

Synonyms

TIMM23, TIMM 23, Mitochondrial import inner membrane translocase subunit Tim23

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Validation Data Gallery

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  WB analysis using 11123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11123-1-AP (Tim23 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 11123-1-AP

  IP result of anti-Tim23 (IP:11123-1-AP, 4ug; Detection:11123-1-AP 1:1000) with mouse heart tissue lysate 1200 ug.

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  IHC staining of human testis using 11123-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11123-1-AP (TIMM23 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11123-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (11123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 124 publications below
• IHC: See 3 publications below
• IF: See 15 publications below
• IP: See 1 publications below

Product Information

11123-1-AP targets Tim23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, monkey, bovine, hamster, megalobrama amblycephala
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Tim23 fusion protein Ag1620
• Full Name: translocase of inner mitochondrial membrane 23 homolog (yeast)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC062707
• Gene Symbol: Tim23
• Gene ID (NCBI): 10431
• RRID: AB_615045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Publications

Species	Application	Title
mouse	WB	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
mouse	WB	Nature; The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.; Authors - Atsushi Hoshino
human	WB	Nature; In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.; Authors - Milica Momcilovic
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
human	WB	Cell Metab; An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.; Authors - Nunu Huang

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