Anticorps Polyclonal de lapin anti-PDI

• Phare
• Validé par KD/KO

PDI Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

47

N° de cat : 11245-1-AP

Synonymes

Cell and organelle markers, DSI, endoplasmic reticulum marker, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase

---

Galerie de données de validation

• 

  WB analysis using 11245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11245-1-AP (PDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11245-1-AP

  WB result of PDI antibody (11245-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells.

• 

  IP experiment of HepG2 using 11245-1-AP

  IP result of anti-PDI(IP:11245-1-AP, 4ug; Detection:11245-1-AP 1:2000) with HepG2 cells lysate 2600 ug.

• 

  IHC staining of human prostate cancer using 11245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11245-1-AP (PDI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11245-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDI antibody (11245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

• 

  FC experiment of HeLa using 11245-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PDI (11245-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, cellules HepG2, tissu cardiaque de souris, tissu cérébral de rat
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 26 publications below
• IHC: See 9 publications below
• IF: See 16 publications below
• FC: See 1 publications below

Informations sur le produit

11245-1-AP cible PDI dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDI Protéine recombinante Ag1747
• Nom complet: prolyl 4-hydroxylase, beta polypeptide
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC014504
• Symbole du gène: P4HB
• Identification du gène (NCBI): 5034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Publications

Species	Application	Title
human,mouse	IHC	Nature; Heart repair by reprogramming non-myocytes with cardiac transcription factors.; Authors - Song Kunhua K
human	IF	J Thromb Haemost; VWF-Gly2752Ser, a novel non-cysteine substitution variant in the CK domain, exhibits severe secretory impairment by hampering C-terminal dimer formation.; Authors - Shuichi Okamoto
	IF	Mol Cell; The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.; Authors - Linyu Sun
human	IHC, WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
mouse	IF	Sci Adv; Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry.; Authors - Katsutoshi Mizuno
mouse	IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C

PDIA6 Polyclonal antibody

18233-1-AP

P4HA3 Polyclonal antibody

23185-1-AP

PRKCSH Polyclonal antibody

12148-1-AP