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  • KD/KO Validated

PRKCSH Polyclonal antibody

PRKCSH Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12148-1-AP


80K H protein, AGE R2, G19P1, Glucosidase 2 subunit beta, Glucosidase II subunit beta, PCLD, PKCSH, PLD1, PRKCSH

Tested Applications

Positive WB detected inA431 cells, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12148-1-AP targets PRKCSH in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKCSH fusion protein Ag2796
Full Name protein kinase C substrate 80K-H
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC013586
Gene Symbol PRKCSH
Gene ID (NCBI) 5589
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.


Product Specific Protocols
WB protocol for PRKCSH antibody 12148-1-APDownload protocol
IHC protocol for PRKCSH antibody 12148-1-APDownload protocol
IF protocol for PRKCSH antibody 12148-1-APDownload protocol
IP protocol for PRKCSH antibody 12148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Exp Mol Med

PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer

Authors - Gu-Choul Shin

Nat Commun

PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway.

Authors - Gu-Choul Shin
  • KD Validated

J Cell Biol

Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins

Authors - Amber M Cheatham

Exp Physiol

AGEs-RAGE axis mediates myocardial fibrosis via activation of cardiac fibroblasts induced by autophagy in heart failure.

Authors - Birong Liang

Cell Rep

Spatial region-resolved proteome map reveals mechanism of COVID-19-associated heart injury.

Authors - Ling Leng

Biochim Biophys Acta

Hepatocystin/80K-H inhibits replication of hepatitis B virus through interaction with HBx protein in hepatoma cell.

Authors - Shin Gu-Choul GC