PDIA6 Polyclonal antibody

• Featured Product
• KD/KO Validated

PDIA6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 18233-1-AP

Synonyms

Protein disulfide isomerase P5, Protein disulfide isomerase A6, P5, ERp5, ER protein 5

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Validation Data Gallery

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  WB analysis using 18233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18233-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18233-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18233-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18233-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18233-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 18233-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18233-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18233-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18233-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18233-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18233-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18233-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 18233-1-AP

  IP result of anti-PDIA6 (IP:18233-1-AP, 4ug; Detection:18233-1-AP 1:3000) with HEK-293 cells lysate 3000ug.

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  IHC staining of human stomach using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 18233-1-AP

  Immunofluorescent analysis of HeLa cells using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18233-1-AP

  Immunofluorescent analysis of HeLa cells using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat kidney tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse lung tissue, HeLa cells, L02 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach tissue, human spleen tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

18233-1-AP targets PDIA6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDIA6 fusion protein Ag12940
• Full Name: protein disulfide isomerase family A, member 6
• Calculated Molecular Weight: 48 kDa, 54 kDa
• Observed Molecular Weight: 48-54 kDa
• GenBank Accession Number: BC001312
• Gene Symbol: PDIA6
• Gene ID (NCBI): 10130
• RRID: AB_10805765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.

Publications

Species	Application	Title
human	IHC	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
mouse	WB	J Exp Med; Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development.; Authors - Jin Huk Choi
human	WB	Nucleic Acids Res; Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.; Authors - Neus Mestre-Farràs ; KD Validated
human	WB	J Immunother Cancer; MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells; Authors - Yoshihiro Morimoto
human	WB,IF,IP	Antiviral Res; Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production; Authors - Lauren Panny