Validation Data Gallery
|Positive WB detected in||rat kidney tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse lung tissue, HeLa cells, L02 cells, HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human stomach tissue, human spleen tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18233-1-AP is PDIA6 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PDIA6 fusion protein Ag12940|
|Full Name||protein disulfide isomerase family A, member 6|
|Calculated molecular weight||48 kDa, 54 kDa|
|Observed molecular weight||48-54 kDa|
|GenBank accession number||BC001312|
|Gene ID (NCBI)||10130|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.
|Product Specific Protocols|
|WB protocol for PDIA6 antibody 18233-1-AP||Download protocol|
|IHC protocol for PDIA6 antibody 18233-1-AP||Download protocol|
|IF protocol for PDIA6 antibody 18233-1-AP||Download protocol|
|IP protocol for PDIA6 antibody 18233-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.
J Exp Med
Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development.
Nucleic Acids Res
Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.
J Immunother Cancer
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Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production