Anticorps Polyclonal de lapin anti-PARK2/Parkin

• Phare
• Validé par KD/KO

PARK2/Parkin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

236

Avis (2)

N° de cat : 14060-1-AP

Synonymes

Parkin, PARK2, Parkinson disease protein 2, Parkin RBR E3 ubiquitin-protein ligase, EC:2.3.2.31

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Galerie de données de validation

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  WB analysis using 14060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 14060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14060-1-AP (PARK2/Parkin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 14060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using PARK2/Parkin antibody (14060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 14060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PARK2/Parkin antibody (14060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PARK2/Parkin antibody (14060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of RAW 264.7 using 14060-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using PARK2/Parkin antibody (14060-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules HEK-293, cellules PC-12, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 211 publications below
• IHC: See 19 publications below
• IF: See 56 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below

Informations sur le produit

14060-1-AP cible PARK2/Parkin dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, Lapin, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PARK2/Parkin Protéine recombinante Ag5092
• Nom complet: Parkinson disease (autosomal recessive, juvenile) 2, parkin
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 42-52 kDa
• Numéro d’acquisition GenBank: BC022014
• Symbole du gène: Parkin
• Identification du gène (NCBI): 5071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing with molecular weights of 24, 31, 36 and 42-52 kDa. Sometimes an additional band of 70 kDa or 110 kDa may be detected, which is caused by ubiquitination modification or formation of Parkin complex (PMID: 10976934, PMID: 18190519).

Publications

Species	Application	Title
	WB	Nat Commun; Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota; Authors - Ya Liu
human	IF	Mol Cell; The HRI branch of the integrated stress response selectively triggers mitophagy; Authors - Yogaditya Chakrabarty
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
mouse	WB	Acta Pharm Sin B; Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy; Authors - Chang Xiao
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
rat	IF	Redox Biol; Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats; Authors - Mengyu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ajte (Verified Customer) (03-08-2022)

It works well for me

• Applications: Western Blot

FH

Elyssa (Verified Customer) (11-11-2019)

This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T47D, MCF7, CAMA1 (only worked in T47D)

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