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UBE2L3 Polyclonal antibody

UBE2L3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 14415-1-AP


E2 F1, L UBC, UBCE7, UBCH7, UbcM4, UBE2L3, Ubiquitin carrier protein L3, Ubiquitin protein ligase L3

Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, K-562 cells, MCF-7 cells, mouse kidney tissue, mouse testis tissue, NIH/3T3 cells, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14415-1-AP targets UBE2L3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2L3 fusion protein Ag5758
Full Name ubiquitin-conjugating enzyme E2L 3
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC053368
Gene Symbol UBE2L3
Gene ID (NCBI) 7332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).


Product Specific Protocols
WB protocol for UBE2L3 antibody 14415-1-APDownload protocol
IHC protocol for UBE2L3 antibody 14415-1-APDownload protocol
IP protocol for UBE2L3 antibody 14415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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