UBC13 Polyclonal antibody

UBC13 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10243-1-AP

Synonyms

UBE2N, UbcH13, UbcH ben, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme N



Tested Applications

Positive WB detected inmouse thymus tissue, human heart tissue, Jurkat cells, mouse small intestine tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10243-1-AP targets UBC13 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBC13 fusion protein Ag0292
Full Name ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC000396
Gene Symbol UBC13/UBE2N
Gene ID (NCBI) 7334
RRIDAB_2288109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Protocols

Product Specific Protocols
WB protocol for UBC13 antibody 10243-1-APDownload protocol
IHC protocol for UBC13 antibody 10243-1-APDownload protocol
IP protocol for UBC13 antibody 10243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyWB

Alzheimers Dement

Suppressing UBE2N ameliorates Alzheimer's disease pathology through the clearance of amyloid beta

Authors - Chen Zhang
mouseWB

Cell Mol Immunol

The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.

Authors - Floriana Mulas
pigWB

Mol Cell Proteomics

The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators

Authors - Nitchakarn Kaokhum
mouseWB,IP

Nat Commun

FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke

Authors - Bin Li
humanWB,IHC

Mol Biomed

SP1 transcriptionally regulates UBE2N expression to promote lung adenocarcinoma progression

Authors - Jianjun Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jessica (Verified Customer) (11-14-2019)

Antibody works well for detecting the endogenous Ubc13 protein in 293T cells. Strong signal and few non specific bands.

  • Applications: Western Blot,
  • Cell Tissue Type: 293T cells