UBC13 Polyclonal antibody

UBC13 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 10243-1-AP

Synonyms

UBE2N, UbcH13, UbcH ben, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme N

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Validation Data Gallery

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  WB analysis of mouse thymus using 10243-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10243-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10243-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10243-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 10243-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10243-1-AP

  IP result of anti-UBC13 (IP:10243-1-AP, 4ug; Detection:10243-1-AP 1:600) with MCF-7 cells lysate 1280ug.

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  IHC staining of human breast cancer using 10243-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP (UBC13 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 10243-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP (UBC13 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 10243-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10243-1-AP (UBC13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse thymus tissue, human heart tissue, Jurkat cells, mouse small intestine tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

10243-1-AP targets UBC13 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBC13 fusion protein Ag0292
• Full Name: ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC000396
• Gene Symbol: UBC13/UBE2N
• Gene ID (NCBI): 7334
• RRID: AB_2288109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.; Authors - Floriana Mulas
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
mouse	WB,IP	Nat Commun; FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke; Authors - Bin Li
human	WB,IHC	Mol Biomed; SP1 transcriptionally regulates UBE2N expression to promote lung adenocarcinoma progression; Authors - Jianjun Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jessica (Verified Customer) (11-14-2019)

Antibody works well for detecting the endogenous Ubc13 protein in 293T cells. Strong signal and few non specific bands.

• Applications: Western Blot,
• Cell Tissue Type: 293T cells