- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-Osteopontin
Osteopontin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (2)
Applications
WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA
Conjugaison
Non conjugué
282
N° de cat : 22952-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HEK-293, cellule C2C12, cellules HepG2, cellules Jurkat, tissu rénal de souris |
Résultats positifs en IHC | tissu rénal de souris, tissu pulmonaire de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HepG2, cellules HEK-293 |
Résultats positifs en FC (Intra) | cellules HepG2, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochimie (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 6 publications below |
WB | See 190 publications below |
IHC | See 62 publications below |
IF | See 74 publications below |
ELISA | See 1 publications below |
CoIP | See 2 publications below |
Informations sur le produit
22952-1-AP cible Osteopontin dans les applications de WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, bovin, Humain, Lapin, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Osteopontin Protéine recombinante Ag19216 |
Nom complet | secreted phosphoprotein 1 |
Masse moléculaire calculée | 314 aa, 35 kDa |
Poids moléculaire observé | 70 kDa, 44-66 kDa |
Numéro d’acquisition GenBank | BC007016 |
Symbole du gène | Osteopontin |
Identification du gène (NCBI) | 6696 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).
Protocole
Product Specific Protocols | |
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WB protocol for Osteopontin antibody 22952-1-AP | Download protocol |
IHC protocol for Osteopontin antibody 22952-1-AP | Download protocol |
IF protocol for Osteopontin antibody 22952-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Circulation Carbonylation of Runx2 at K176 by 4-Hydroxynonenal Accelerates Vascular Calcification | ||
Adv Mater Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon-Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects | ||
Cell Stem Cell Decoding the temporal and regional specification of microglia in the developing human brain. | ||
Bioact Mater Urchin-like multiscale structured fluorinated hydroxyapatite as versatile filler for caries restoration dental resin composites | ||
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Shinford (Verified Customer) (10-26-2023) | The WB bands exhibit remarkable strength and clarity, with various treatments leading to significant changes in expression.
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FH Shinford (Verified Customer) (09-24-2023) | This antibody works well in esophageal cancer and lung cancer cell lines, and the detection antibody band is clear. It is recommended to use a diluted concentration of 1:2000.
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FH Elisa (Verified Customer) (08-16-2023) | Tested on mouse skull sections, with appropriate blocking: give a nice stain of osteopontin, but maybe it can be optimized to be able to use an higher dilution.
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FH Davide (Verified Customer) (09-20-2022) | The antibody works really well. Fantastic product, and the possibility to try the product with the free sample is something really appreciated. Will surely buy a full vial soon.
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FH WEI (Verified Customer) (03-08-2022) | Strong specific Bands
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FH LUNFENG (Verified Customer) (01-27-2020) | VERY GOOD
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FH Jie (Verified Customer) (01-23-2020) | Works with one specific band and one faint non-specific band. However, can be distinguished by protein size. So works fine in general.
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FH Jingwen (Verified Customer) (01-17-2020) | This antibody showed the specific band and staining results in both western blot and immunofluorescence staining. I strongly recommend it.
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FH Barbara (Verified Customer) (09-28-2019) | Fluorescence-based detection of OPN (green) expression in paraffin-embedded human aortic tissue, heat mediated antigen retrival with 10mM citrate buffer (pH 6,0)
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