• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-Osteopontin

Osteopontin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 22952-1-AP

Synonymes

SPP1, PSEC0156, OPN, ETA 1, BSPI



Applications testées

Résultats positifs en WBcellules HEK-293, cellule C2C12, cellules HepG2, cellules Jurkat, tissu rénal de souris
Résultats positifs en IHCtissu rénal de souris, tissu pulmonaire de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2, cellules HEK-293
Résultats positifs en FC (Intra)cellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochimie (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

22952-1-AP cible Osteopontin dans les applications de WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, bovin, Humain, Lapin, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Osteopontin Protéine recombinante Ag19216
Nom complet secreted phosphoprotein 1
Masse moléculaire calculée 314 aa, 35 kDa
Poids moléculaire observé 70 kDa, 44-66 kDa
Numéro d’acquisition GenBankBC007016
Symbole du gène Osteopontin
Identification du gène (NCBI) 6696
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).

Protocole

Product Specific Protocols
WB protocol for Osteopontin antibody 22952-1-APDownload protocol
IHC protocol for Osteopontin antibody 22952-1-APDownload protocol
IF protocol for Osteopontin antibody 22952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Circulation

Carbonylation of Runx2 at K176 by 4-Hydroxynonenal Accelerates Vascular Calcification

Authors - Xiaoxuan Zhai
mouseIF

Adv Mater

Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon-Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects

Authors - Congyang Mao
humanIF

Cell Stem Cell

Decoding the temporal and regional specification of microglia in the developing human brain.

Authors - Yanxin Li
rat,humanIF

Bioact Mater

Urchin-like multiscale structured fluorinated hydroxyapatite as versatile filler for caries restoration dental resin composites

Authors - Shuning Zhang
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
mouseIF

Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (10-26-2023)

The WB bands exhibit remarkable strength and clarity, with various treatments leading to significant changes in expression.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Lung cancer cell lines and lung cancer tissues with different treatment
Osteopontin Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines and lung cancer tissues with different treatment (Cat no:22952-1-AP)
FH

Shinford (Verified Customer) (09-24-2023)

This antibody works well in esophageal cancer and lung cancer cell lines, and the detection antibody band is clear. It is recommended to use a diluted concentration of 1:2000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cancer cell lines
Osteopontin Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:22952-1-AP)
FH

Elisa (Verified Customer) (08-16-2023)

Tested on mouse skull sections, with appropriate blocking: give a nice stain of osteopontin, but maybe it can be optimized to be able to use an higher dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse skull
FH

Davide (Verified Customer) (09-20-2022)

The antibody works really well. Fantastic product, and the possibility to try the product with the free sample is something really appreciated. Will surely buy a full vial soon.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocytes
Osteopontin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocytes (Cat no:22952-1-AP)
FH

WEI (Verified Customer) (03-08-2022)

Strong specific Bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart
FH

LUNFENG (Verified Customer) (01-27-2020)

VERY GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Works with one specific band and one faint non-specific band. However, can be distinguished by protein size. So works fine in general.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney
FH

Jingwen (Verified Customer) (01-17-2020)

This antibody showed the specific band and staining results in both western blot and immunofluorescence staining. I strongly recommend it.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
  • Cell Tissue Type: Mouse heart and cultured cardiomyocytes.
FH

Barbara (Verified Customer) (09-28-2019)

Fluorescence-based detection of OPN (green) expression in paraffin-embedded human aortic tissue, heat mediated antigen retrival with 10mM citrate buffer (pH 6,0)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: aorta
Osteopontin Antibody Immunohistochemistry, validation (1:150 dilution) in aorta (Cat no:22952-1-AP)